Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ribosomal architecture and rRNA modification landscape in the tick-borne parasite Babesia divergens

ABSTRACT: Babesia are tick-borne intracellular apicomplexan parasites that infect a wide range of wild and domestic animals (e.g., cattle), resulting in significant economic losses to the livestock industry. Humans are considered accidental hosts for a few Babesia species. Babesia microti and B. divergens are the most prevalent causes of human babesiosis that are showing a broadening geographic distribution. Due to the complex life cycle of Babesia species, their survival depends on the precise control of gene expression, which is primarily regulated by epigenetic, transcriptional, and post-transcriptional mechanisms. High-resolution structural information on key components of the translation machinery, such as ribosomes, could aid in the development of antiparasitic drugs. Here, we report cryogenic electron microscopy ribosome structures from Babesia divergens, showing associated tRNAs, an mRNA fragment, and RACK1, a signaling scaffold crucial to translation regulation. Density map analysis displays ribosome regions at high resolution, which, when combined with nanopore sequencing, enabled the comprehensive identification of rRNA modifications, including modifications unreported in other organisms. The rRNA modifications localize not only to the reduced B. divergens rRNA expansion segments but also to functionally essential ribosomal sites.

INSTRUMENT(S):

ORGANISM(S): Babesia Divergens

SUBMITTER: Cristina Gutierrez-Vargas

LAB HEAD: Melissa Leger-Abraham

PROVIDER: PXD069329 | Pride | 2026-06-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	220310L_CVSAM09937_BdRibo-CT.raw	Raw
	220310L_CVSAM09937_BdRibo-CT_Piroplasma_Vargas.mzTab	Mztab
	220310L_CVSAM09937_BdRibo-CT_Piroplasma_Vargas.pdResult	Other
	PiroplasmaDB-56_Bdivergens1802A_AnnotatedProteins.fasta	Fasta
	checksum.txt	Txt

Items per page:

1 - 5 of 5

Similar Datasets

Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

Project description:Human babesiosis, especially caused by the cattle derived Babesia divergens parasite, is on the increase, resulting in renewed attentiveness to this potentially life threatening emerging zoonotic disease. The molecular mechanisms underlying the pathophysiology and intra-erythrocytic development of these parasites are poorly understood. This impedes concerted efforts aimed at the discovery of novel anti-babesiacidal agents. By applying sensitive cell biological and molecular functional genomics tools, we describe the intra-erythrocytic development cycle of B. divergens parasites from immature, mono-nucleated ring forms to bi-nucleated paired piriforms and ultimately multi-nucleated tetrads that characterizes zoonotic Babesia spp. This is further correlated for the first time to nuclear content increases during intra-erythrocytic development progression, providing insight into the part of the life cycle that occurs during human infection. High-content temporal evaluation elucidated the contribution of the different stages to life cycle progression. Moreover, molecular descriptors indicate that B. divergens parasites employ physiological adaptation to in vitro cultivation. Additionally, differential expression is observed as the parasite equilibrates its developmental stages during its life cycle. Together, this information provides the first temporal evaluation of the functional transcriptome of B. divergens parasites; information that could be useful in identifying biological processes essential to parasite survival for future anti-babesiacidal discoveries.

2015-04-10 | GSE67677 | GEO

Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

2015-04-10 | E-GEOD-67677 | biostudies-arrayexpress

Proteomic characterization of Babesia divergens extracellular vesicles

Project description:Protozoan parasites of the genus Babesia are considered a serious threat to humans and animals worldwide. Babesia parasites are naturally transmitted by ticks and infect the erythrocytes or red blood cells (RBCs) of many vertebrates causing babesiosis. Infectious species include B. divergens, which causes asymptomatic to fatal babesiosis in humans and red water fever in cattle in Europe. To better understand the biology of B. divergens and to develop diagnostic and control strategies for babesiosis, we studied the extracellular vesicles (EVs) released into the environment by B. divergens-infected red blood cells (iRBCs). B. divergens parasites were cultured in human erythrocytes in vitro in a complete medium containing low human vesicle (LHV) serum. Uninfected erythrocytes (uRBC) were also maintained in the LHV complete medium. Bd-derived EVs and uRBC-derived EVs were collected and purified from the supernatants of B. divergens and uRBC cultures, respectively. Proteins were extracted from Bd-derived EVs and uRBC-derived EVs.

2025-07-22 | PXD052589 | Pride

Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

2023-12-31 | GSE243493 | GEO

Babesia divergens strain:1802A

Project description:Babesia divergens strain:1802A Genome sequencing and assembly

| PRJNA230984 | ENA

Comparison of high throughput RNA sequences between Babesia bigemina and Babesia bovis revealed consistent differential gene expression that is required for Babesia life cycle in the vertebrate and invertebrate hosts.

Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites

2022-12-20 | GSE214115 | GEO

Babesia divergens

Project description:Morphological and molecular descriptors of the developmental cycle of Babesia divergens parasites in human erythrocytes

| PRJNA280620 | ENA

Comparative transcriptome analysis of geographically distinct virulent and attenuated Babesia bovis

Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation. Pair end RNA-sequencing reads on three biological replicate sample pairs of virulent parent and attenuated derivative Babesia bovis strain isolated in Argentina.

2013-10-23 | E-GEOD-51560 | biostudies-arrayexpress

Babesia microti isolate:Babesia

Project description:Babesia microti isolate:Babesia Genome sequencing

| PRJNA1212846 | ENA

Differential transcriptional expression by Babesia bovis during parasite development within bovine erythrocytes and tick hemolymph

Project description:Babesia parasites transition between a mammalian host, where they cause babesiosis, and the tick vector that transmits them. This transition provides an environmental signal resulting in altered gene expression allowing the completion of the parasite’s life cycle. A comparison of the different life stages that occur within mammalian and tick hosts can provide insight into the adaptation of Babesia to these different environments. In this study, we used RNA-Seq to compare gene expression between Babesia bovis blood stages and tick derived kinetes.

2020-10-21 | GSE144066 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data