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Mouse serum DIA-based proteomic profiling after 1-day or 7-day daily heat stress exposure

ABSTRACT: This project profiles heat stress (HS)-induced alterations in the mouse serum proteome to identify circulating proteins that are consistently regulated by HS. Mice were assigned to three groups: PF (pair-fed control at room temperature, RT), HS1D (daily HS for 1 day, 6 h/day), and HS7D (daily HS for 7 consecutive days, 6 h/day). The PF group serves as the shared control and was collected at the same time point as the HS1D group (RT, 1 day), providing a reference for HS-associated changes. The primary objective is to identify overlapping proteomic changes in both HS groups relative to PF, highlighting robust HS-responsive circulating proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Blood Serum

SUBMITTER: Xu Feng

LAB HEAD: Xiang-Hang Luo

PROVIDER: PXD074893 | Pride | 2026-04-28

REPOSITORIES: Pride

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			Action	DRS
	HS1D1.raw	Raw
	HS1D2.raw	Raw
	HS1D3.raw	Raw
	HS7D1.raw	Raw
	HS7D2.raw	Raw

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Publications

A skin-hypothalamus axis couples heat stress and metabolic dysfunction.

Zhou Hai-Yan HY Feng Xu X Wen Jie J Xiao Yao Y Wang Li-Wen LW Chen Lin-Yun LY Xie Gen-Qing GQ Zhao Jia-Jun JJ Huang Yan Y Luo Xiang-Hang XH

Cell 20260421

With the ongoing rise in global temperatures, the prevalence of heat-stress-related chronic health disorders has increased. However, whether heat stress has an enduring impact on metabolic health remains unclear. Here, we report that mice exposed to heat stress were more susceptible to metabolic dysfunction upon subsequent exposure to an obesogenic diet. Upon heat stress, we found that elevated skin-derived kallikrein-related peptidase 14 (KLK14) imprinted hypothalamic LRRC7<sup>+</sup> astrocyt ...[more]

PMID: 42019490

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A skin-hypothalamus axis couples heat stress and metabolic dysfunction

Project description:With the ongoing rise in global temperatures, heat stress is increasingly implicated in chronic metabolic disorders; however, whether a transient high-temperature experience leads to enduring metabolic vulnerability and persistent hypothalamic adaptations remains unclear. To address this question, we established a controlled heat stress (HS) mouse model. Eight-week-old male mice with comparable body weights were assigned to HS or paired-fed (PF) conditions. HS mice were exposed to 37 °C for 6 h during the dark phase for 1 week with ad libitum access to food. PF mice were maintained at room temperature and pair-fed to match the food intake consumed by HS mice during the 6-h exposure window, followed by ad libitum feeding for the remaining 18 h to ensure comparable total daily intake. After HS, mice were returned to room temperature for a 2-week quiescent stage (QS), during which blood glucose and serum corticosterone normalized, indicating recovery from acute stress. Hypothalamic cells were collected from PF, HS, and QS groups for single-cell RNA sequencing to characterize heat stress–associated changes in hypothalamic cellular composition and transcriptional states. We observed a sustained increase in astrocyte abundance after HS that persisted into QS, and identified an expanded astrocyte subpopulation marked by high Lrrc7 expression, providing a foundation for further investigation of persistent hypothalamic responses to heat exposure in the context of metabolic regulation.

2026-04-20 | GSE322787 | GEO

Molecular and cellular adaptations of the mammary gland regulating milk constitutes and immunity of heat stressed dairy cows

Project description:The molecular and cellular mechanisms underlying the adaption of the mammary gland (MG) to increased ambient heat, and which account for changes in milk composition and immunity are not known yet. This study aimed to elucidate the expression of MG proteins and differences in milk composition and ruminal short-chain fatty acid concentrations of dairy cows experiencing 7 days of heat stress (28°C, THI = 76), pair-feeding (PF) or ad libitum feeding (CON) at thermoneutrality (16°C, THI = 60; each n=10). The feed intake of PF cows were matched to the intake of HS cows. The portion of ruminal acetate, the acetate:propionate ratio, and milk urea concentration were greater whereas milk protein yield and lactose yield were lower in HS than CON cows on day 3 and 6 of the challenge, whilst energy and fat corrected milk were lower in HS than CON and PF cows. The proteome analysis revealed induced bacterial invasion of epithelial cells, leukocyte transendothelial migration, reduction of the pyruvate and carbon metabolism, and platelet activation in the MG of HS compared to CON and/or PF cows. These results highlight adaptive metabolic and immune responses to mitigate the negative effects of ambient heat in the mammary gland.

2025-05-06 | PXD048796 | Pride

Daily cycling of genome-wide histone methylation

Project description:Daily (circadian) clocks are essential for regulating and coordinating physiology in mammals. Modulation of local chromatin structure has been shown for only a small number of genes, principally thought to be directly modulated by Clock-mediated histone acetylation. Here we show that genome-wide changes of active and suppressive histone marks control global chromatin architecture over the day and night.

2013-01-01 | GSE37393 | GEO

Persistent changes in the glutamatergic and GABAergic signaling in adolescent rats exposed developmentally to chlorpyrifos

Project description:Male Sprague Dawley rats were treated daily by oral gavage with either corn oil, chlorpyrifos, or PF-04457845 (selective FAAH inhibitor) from postnatal day10-16. The rats were scarified on postnatal day 38, and brains were collected and stored at -80˚C. Amygdala was isolated from the brain, homogenized the tissue, proteins were quantified, trypsin digested, and analyzed by LC ESI MS/MS.

2022-08-11 | PXD017405 | Pride

CRYPTOCHROMES promote daily proteome homeostasis

Project description:The daily organisation of most mammalian cellular functions is attributed to circadian regulation of clock-controlled protein expression, driven by daily cycles of CRYPTOCHROME-dependent transcriptional feedback repression. To test this, we used quantitative mass spectrometry to compare wild type and CRY-deficient fibroblasts under constant conditions. In CRY-deficient cells, we found that temporal variation in protein, phosphopeptide, and K+ abundance was at least as great as wild type controls. Most strikingly. the extent of temporal variation within either genotype was much smaller than overall differences in proteome composition between WT and CRY-deficient cells. This proteome imbalance in CRY-deficient cells and tissues was associated with increased susceptibility to proteotoxic stress, which impairs circadian robustness, and may contribute to the wide-ranging phenotypes of CRY-deficient mice. Rather than generating largescale daily variation in proteome composition, we suggest it is plausible that the various transcriptional and post-translational functions of CRY proteins ultimately act to promote protein and osmotic homeostasis against daily perturbation.

2021-11-19 | PXD019499 | Pride

The role of PER1/2 and feeding rhythms in daily oscillations of mitochondrial nutrient utilization and rate-limiting enzymes

Project description:Mitochondria are major suppliers of cellular energy through nutrients oxidation. Little is known about the mechanisms that enable mitochondria to cope with changes in nutrient supply and energy demand that naturally occur throughout the day. To address this question, we applied mass-spectrometry based quantitative proteomics on isolated mitochondria from mice sacrificed throughout the day and identified extensive oscillations in the mitochondrial proteome. We found that rate-limiting mitochondrial enzymes that process lipids and carbohydrates accumulate in a diurnal manner and dependent on the clock proteins PER1/2. In this conjuncture, we uncovered daily oscillations in mitochondrial respiration that peak during different times of the day in response to different nutrients. Notably, the diurnal regulation of mitochondrial respiration was blunted in mice lacking PER1/2 or upon nutritional challenge such as high fat diet. We propose that the clock proteins PER1/2 optimize mitochondrial metabolism to daily changes in energy supply/demand and thereby serve as a rheostat for mitochondrial nutrient utilization.

2018-10-17 | PXD001732 | Pride

CELSeq2-profiles for siRNA screening and daily timecourse for early reprogramming to pluripotency.

Project description:AIM: To find molecular signatures associated to the siRNA-mediated knockdowns in order to be able to identify similarities among different knockdowns. DESCRIPTION: Each sample includes biological triplicates for 35 siRNA-mediated knockdowns targeting 30 chromatin-associated proteins during in early reprogramming to iPS at day 6. A daily timecourse from reprogramming cells, without treatment from MEFs until day 6 is also included in triplicate.

2019-03-14 | GSE118679 | GEO

Daily cycling of genome-wide histone methylation

2013-01-01 | E-GEOD-37393 | biostudies-arrayexpress

Daily ocean time-series

Project description:Daily ocean time-series 2011 at San Pedro Ocean time-series

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Data independent acquisition mass spectrometry (DIA-MS) analysis of FFPE rectal cancer samples offers in depth proteomics characterization of response to neoadjuvant chemoradiotherapy

Project description:A total of 97 LARC patients treated at the Institute for Oncology and Radiology of Serbia in the period of 2018-2019 were included in the study. Patients were treated with long-course chemoradiotherapy (CRT): Radiotherapy (RT) was delivered with a total dose of 50.4 Gy in 28 fractions; concomitant chemotherapy (5-FU, 350 mg/m2 daily) and Leucovorin (25 mg/m2 daily) was administered during the first and the fifth week of RT. Patients were evaluated in week 6-8 after treatment completion with pelvic MRI scan and rigid proctoscopy. Pathohistological response after surgery was assessed according to tumor regression grading (TRG) categories by Mandard. Twenty biopsy samples taken at diagnosis were used for proteomic analysis, 9 responders (R, TRG 1-2), and 11 non-responders (NR, TRG 3-5), in order to achieve the maximum range of different molecular features potentially associated with response.

2023-11-21 | PXD040451 | Pride

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