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Homo sapiens

ABSTRACT: Transcriptomic profiling of MAFB-KD THP-1 macrophages infected with Mycobacterium tuberculosis

PROVIDER: PRJDB13594 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR375630_1.fastq.gz	Fastqsanger.gz
	DRR375630_2.fastq.gz	Fastqsanger.gz
	DRR375631_1.fastq.gz	Fastqsanger.gz
	DRR375631_2.fastq.gz	Fastqsanger.gz
	DRR375632_1.fastq.gz	Fastqsanger.gz

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The association and difference of normal THP-1 cell and H37Rv infected THP-1 cell.

Project description:①Background:Tuberculosis is mainly a respiratory tract infection caused by mycobacterium tuberculosis and one of the leading causes of death worldwide. According to the Global Tuberculosis Report in 2021, About a quarter of the world's population is infected with Mycobacterium tuberculosis and China is the second highest burden of TB. Although TB diagnosis and prevention techniques have become more mature, the number of TB cases is still increasing, mainly due to: the prevalence of drug-resistant tuberculosis bacteria, tuberculosis and HIV co-infection, long incubation time of mycobacterium tuberculosis difficult to early diagnosis and so on. Therefore, it is of great significance to study the pathogenesis of mycobacterium tuberculosis infection.②Method: THP-1 cells were treated with 50ng/ml PMA for 24 hours, so that THP-1 cell can be induced into macrophages. After that THP-1 macrophages were infected with mycobacterium tuberculosis H37Rv(MOI=1), which were collected and applied to RNA-sequencing. The constructed sequencing library was sequenced using an Illumina Novaseq 6000 system.

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Project description:This study aims to identify the specific miRNA of mycobacterium tuberculosis (M.tb) infected THP-1 by next-generation sequencing, and further to explore the role of miRNA in innate immunity against M.tb infection.Comprehensive analysis of the next-generation sequencing results showed that the expression of miR-99a-5p was significantly lower in the MTB infected THP-1 cells.

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Project description:Purpose: The goals of this study are systematic analysis of cellular pathway in THP-1 Macrophage which infected with wild type mycobacterium smegmatis (Wt) or MSMEG_5447 gene defected Mycobacterium smegmatis( ΔM5447) at 24h postinfection. Method: THP-1 Macrophages mRNA profiles infected with Wt orΔM5447 at 24h postinfection were generated by deep sequencing, in duplicate, using Illumina HiSeq platform and human transcriptome (RefSeq transcriptome index hg19) is a reference sequence. Results: In total, 497 differentially expressed genes (DEGs) was identified in ΔM5447-infected macrophages relative to the Wt-infected macrophages which including 173 up-regulated genes and 324 down-regulated genes using adjusted p-value<0.05 as the threthold criteria. The DEGs of THP-1 macrophages infected with ΔM5447 strain was enriched in inflammatory related pathway. Conclusion: Our study find that the THP-1 macrophages infected with ΔM5447 strain display decreased inflammatory response, MSMEG_5447 may play an essential role in inflammatory response.

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