Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Chlamydomonas reinhardtii

ABSTRACT: RNA-seq analysis for Chlamydomonas in ccm1 and cbp1 mutant

PROVIDER: PRJDB17792 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR544695_1.fastq.gz	Fastqsanger.gz
	DRR544695_2.fastq.gz	Fastqsanger.gz
	DRR544696_1.fastq.gz	Fastqsanger.gz
	DRR544696_2.fastq.gz	Fastqsanger.gz
	DRR544697_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 192

Similar Datasets

Chlamydomonas reinhardtii

Project description:RNA-seq analysis for Chlamydomonas in lcr1 mutant

| PRJDB17220 | ENA

Chlamydomonas reinhardtii

Project description:RNA-seq analysis for Chlamydomonas in saga1 mutant

| PRJDB15122 | ENA

Small RNAs in Chlamydomonas reinhardtii

Project description:RNA populations in Chlamydomonas reinhardtii Keywords: Highly parallel pyrosequencing

2007-05-16 | GSE7308 | GEO

Transcriptomics analysis of the Chlamydomonas reinhardtii diurnal cycle.

Project description:Here, we report a transcriptomics analysis on a day in the life of Chlamydomonas reinhardtii. Cultures of this unicellular alga were grown in photobioreactors on a 12 h light / 12 h dark cycle. Samples were collected at regular intervals and subjected to a transcriptomics analysis by RNA-Seq.

2019-01-03 | GSE112394 | GEO

Chlamydomonas reinhardtii strain:C9

Project description:RNA-seq analysis for Chlamydomonas in H82 (cas mutant)

| PRJDB4595 | ENA

Transcriptome analysis of Chlamydomonas reinhardtii chloroplast and mitochondria

Project description:Here, we report on the transcriptome of the organelles of the micro-alga, Chlamydomonas reinhardtii, sampled under a number of different conditions. The preparation of the RNA-Seq libraries and their analysis were performed using protocols optimized for organellar transcripts. Samples include growth in media +/– Fe, growth in media +/– Cu, diurnal growth samples collected in dark and light, and the sexual cycle.

2017-11-27 | GSE101944 | GEO

divergence in basal transcriptomes of "Wild Type" Chlamydomonas reinhardtii strains

Project description:This is a study to compare the basal transcriptomes of several widely used laboratory strains of the Chlorophyte alga, Chlamydomonas reinhardtii. Given that there is a high degree of genetic diversity among the closely-related laboratory strains, we wished to examine how much variation there is at the transcriptome level. A panel of WT strains (CC-124, CC-125, CC-1009, CC-1690, CC-1691), all believed to be descended from a single zygospore isolated in 1945, were chosen based on their representing the oldest lineages among the standard laboratory strains. Additionally, CC-4532, which was the source for the current (v6) reference assembly, and CC-4533, which is the initial parental strain of the CLiP library collection of mutant strains, were also included in this study based on their significance to the Chlamydomonas community. All strains were grown in liquid cultures under identical, mixotrophic conditions (light + acetate) to mid-log phase before collecting mRNA for RNA-Seq analysis.

2025-11-05 | GSE296941 | GEO

Zinc deficiency impacts CO2 assimilation and disrupts copper homeostasis in Chlamydomonas reinhardtii

Project description:Zinc is an essential nutrient because of its role in catalysis and in stabilizing protein structure, but excess zinc can also be deleterious. Four nutritional zinc states have been identified in the alga Chlamydomonas reinhardtii: zinc toxic, zinc replete, zinc deficient and zinc limited. Growth is inhibited in zinc-limited and zinc toxic cells relative to zinc-replete cells, while zinc-deficiency is visually asymptomatic but distinguished by the accumulation of transcripts encoding ZIP family transporters. To identify targets of zinc deficiency and mechanisms of zinc acclimation, we used RNA-seq to probe zinc nutrition responsive changes in gene expression. We identified a subset of genes encoding zinc-handling components, including ZIP family transporters and candidate zinc chaperones. In addition, we noted an impact on two other regulatory pathways, the carbon concentrating mechanism (CCM) and the nutritional copper regulon. Targets of transcription factor Ccm1 and various CAH genes are up-regulated in zinc-deficiency, as a likely consequence of reduced carbonic anhydrase activity, which is validated by mass spectrometry and immunoblot analysis of Cah1, Cah3 and Cah4. Chlamydomonas is therefore not able to grow photoautotrophically in air in zinc limiting conditions, but supplementation with 1% CO2 restores growth to wild-type rates, suggesting that the inability to maintain CCM is a major consequence of zinc limitation. Surprisingly, we noted also that the Crr1 regulon, which responds to Cu limitation, is also turned on in zinc deficiency, and in fact, Crr1 is required for growth in zinc-limiting conditions. Zinc deficient cells are functionally copper deficient, as evidenced by reduced plastocyanin abundance, even though they hyperaccumulate copper up to 50-fold over normal levels. We suggest that zinc-deficient cells sequester Cu in a bio-unavailable form, perhaps to prevent mis-metallation of critical zinc sites.

2013-05-03 | GSE41096 | GEO

Small RNAs in Chlamydomonas reinhardtii

Project description:RNA populations in Chlamydomonas reinhardtii Keywords: Highly parallel pyrosequencing Small RNAs were prepared from Chlamydomonas reinhardtii total extracts,ligated to a 3' adaptor and a 5' acceptor sequentially, and then RT-PCR amplified. PCR products were reamplified using a pair of 454 cloning primers and provided to 454 Life Sciences (Branford, CT) for sequencing. For technical details, see Tao Zhao, Guanglin Li, Shijun Mi, Shan Li, Gregory J. Hannon, Xiu-Jie Wang, and Yijun Qi. 2007. A Complex System of Small RNAs in the Unicellular Green Alga Chlamydomonas reinhardtii. Genes & Development

2007-05-15 | E-GEOD-7308 | biostudies-arrayexpress

Chlamydomonas reinhardtii strain:C9

Project description:RNA-seq for Chlamydomonas tar1-1 mutant

| PRJDB3021 | ENA