Project description:Here, we report a transcriptomics analysis on a day in the life of Chlamydomonas reinhardtii. Cultures of this unicellular alga were grown in photobioreactors on a 12 h light / 12 h dark cycle. Samples were collected at regular intervals and subjected to a transcriptomics analysis by RNA-Seq.
Project description:Chloroplast phosphate translocators coordinate carbon allocation between the cytosol and plastid during heterotrophic growth. Here, RNA-seq was performed to characterize transcriptome reprogramming in Chlamydomonas reinhardtii following transfer from low light to extended darkness, comparing wild type (CC-4532 background) and an independent CreTPT10 knockout mutant (tpt10b).
Project description:Here, we report on the transcriptome of the organelles of the micro-alga, Chlamydomonas reinhardtii, sampled under a number of different conditions. The preparation of the RNA-Seq libraries and their analysis were performed using protocols optimized for organellar transcripts. Samples include growth in media +/– Fe, growth in media +/– Cu, diurnal growth samples collected in dark and light, and the sexual cycle.