Project description:Histones were isolated from brown adipose tissue and liver from mice housed at 28, 22, or 8 C. Quantitative top- or middle-down approaches were used to quantitate histone H4 and H3.2 proteoforms. See published article for complimentary RNA-seq and RRBS datasets.
Project description:In this study, we performed ChIP-seq of RNA polymerase II, phosphorylated RNA polymerase II and related epigenetic modifications to elucidate transcriptional activities of mouse oocytes and preimplantation embryos. Through analyzing epigenetic alterations of transgenically-modified growing oocytes, early embryos from aged female, and early embryos treated with drugs disturbing proteostasis, we identified critical roles of proteostasis in transcriptional regulation of mouse oocytes and early embryos.