Project description:In mammalian systems, extracellular small RNAs can operate in a paracrine manner to communicate information between cells, relying on transport within vesicles. “Foreign” small RNAs derived from bacteria, plants and parasites have also been detected in mammalian body fluids, sparking interest in whether these could mediate inter-species communication. However, there is no mechanistic framework for RNA-mediated interspecies communication and the active movement of RNA via vesicles has not been shown outside of mammals. Here we demonstrate that specific microRNAs and Y RNAs are packaged into vesicles secreted by a gastrointestinal nematode, Heligmosomoides polygyrus, which naturally infects mice. Total RNA was extracted from the serum of mice infected with Litomosoides sigmodontis at 60 days post infection
Project description:We collected whole genome testis expression data from hybrid zone mice. We integrated GWAS mapping of testis expression traits and low testis weight to gain insight into the genetic basis of hybrid male sterility.
Project description:Effect of voluntary exercise training on microRNA sequencing profile in secretory extracellular vesicles derived from skeletal muscle of mice
Project description:To investigate changes in serum extracellular vesicle miRNAs during adipose tissue regeneration, we created tamoxifen-inducible adipocyte-specific insulin receptor knockout (iFIRKO) mice. We then performed comprehensive miRNA analysis on the serum extracellular vesicles of iFIRKO and control mice.
