Project description:To identify the key microRNAs (miRNAs) of hMSCs required for fate determination, miRNA profiling was performed with hMSCs from three different sources including adipose-derived stem cells (ADSCs), bone-marrow-derived stem cells (BMSCs), and umbilical cord-derived stem cells (UCSCs) versus fibroblasts, a more differentiated mesenchymal cell types. We compared the expression profiles of two different donors per hMSCs to that of fibroblasts. All hMSCs were used for profiling at passage 3-6.
Project description:To identify the key microRNAs (miRNAs) of hMSCs required for fate determination, miRNA profiling was performed with hMSCs from three different sources including adipose-derived stem cells (ADSCs), bone-marrow-derived stem cells (BMSCs), and umbilical cord-derived stem cells (UCSCs) versus fibroblasts, a more differentiated mesenchymal cell types.
Project description:Adipose tissue is a potential site of accumulation of toxicants, including trace elements, which can have an impact on tissue functionality. To investigate the molecular perturbations induced by Cd in mature adipocytes, we treated Adipose Tissue-derived human mesenchymal stem cells (AD-hMSCs) with 1microM cadmium for 48h and we used microarrays to detail the global program of gene expression.
Project description:Proteome experiment was peformed on exosomes of human minor salivary gland mesenchymal stem cells and adipose-derived stem cells to find out the same points and difference of these two kinds of exosomes, which can hopefully give further guidance on further therapy research
Project description:Mesenchymal stem cells (MSCs)-derived exosomes (exo) have shown comprehensive application prospects over the years. Despite similar functions, exomes from different origins present heterogeneous characteristics and components; however, there are no relevant proteomic analyses. In this study, we isolated exosomes from MSCs, derived from different tissues, by ultracentrifugation. A total of 1014 proteins were detected using a label-free method and analyzed with bioinformatics tools. The results revealed their shared function in the extracellular matrix receptor. Bone marrow-MSCs-derived exosomes showed superior regeneration ability. Likewise, adipose tissue-MSCs-derived exosomes played a significant role in immune regulation. Whereas, umbilical cord-MSCs-derived exosomes were more prominent in tissue damage repair.
Project description:Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue of patients with obesity or healthy controls and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq). We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in human adipose-derived MSCs.
Project description:Adult Mesenchymal stem cells (MSCs) derived exosomes have recently gained importance as a cell-free therapy for several chronic and inflammatory diseases. It is believed that these exosomes contain several biologically active molecules (i.e. miRNAs, proteins) which help to cure the diseases. The majority of published reports to date that have investigated the secretome of MSCs have done so using canonical expansion of cell culture conditions. However, the microenvironment experience by MSC post administration into animal models and patients is strikingly different. Thus instead of using secretome, Exosomes purified from adipose tissue (AD), Wharton Jelly (WJ) and Bone marrow BM) are widely studied. However, the content of the exosomes may differ based on their sources from where they are originated. It remains unclear, however, which types of proteins are packaged into exosomes compared with the cells from which they are derived. To explore these exosomes as a cell-free therapy for specific diseases, it is therefore, important to get comprehensive knowledge of the bioactive molecules present in the exosomes. In this study, we have purified the exosomes from three different sources (AD, WJ, and BM) and using RNA-Seq approach, we have categorized the common and different microRNAs present in the exosomes.
