Project description:Genomic safe harbors (GSHs) are utilized as an ideal integration site for generating transgenic organisms and cells. Discovery of GSHs is one of the crucial factors for the advancement of basic and applied biology in the species. Such GSHs were discovered in Pv11 (Polypedilum vanderplanki) cell line, which can survive extreme desiccation. To identify the integration sites, high-molecular-weight genomic DNAs were extracted. The DNA libraries were prepared and sequenced with a Nanopore MinION sequencer. In the way to confirm that GSHs loci are localized in open chromatin regions we prepared ATAC-seq libraries, which were sequenced in Illumina HiSeq2500.
2022-03-01 | GSE190481 | GEO
Project description:Bacterial whole-genome read data from the Oxford Nanopore Technologies MinION™ nanopore sequencer.
Project description:Evaluation of PCR conditions for characterizing bacterial community with full-length 16S rRNA genes using a portable Nanopore sequencer
| PRJDB9684 | ENA
Project description:DNA Methylation classification on the Oxford Nanopore Technologies MinION sequencer
| PRJNA317271 | ENA
Project description:Comparison of Nine Extraction Methods for Bacterial Identification Using the ONT MinION Sequencer
Project description:We performed direct cDNA sequencing in HeLa GFP∆Promoter cells by Oxford Nanopore Technology (ONT) on a MinION device to detect EGFP RNA levels after DSB induction.