Project description:The aim of this study was to investigate the response in gene expression before and after exposure to the Benzimidazoles drug flubendazole in adult female Ascaridia galli worms. The nematode Ascaridia galli (order Ascaridida) is an economically important intestinal parasite responsible for increased food consumption, reduced performance, and mortality in commercial poultry production. Parasite control relies on repeated use of dewormers (anthelmintics). Benzimidazoles are currently the only anthelmintic registered against A. galli in the EU and there is an obvious risk that overuse of one drug class may lead to resistance. The worms were collected from a commercial laying hen farms before and on day three during a treatment period of 7 days with flubendazole.
Project description:Ascaridia galli (A. galli) is one of the most common intestinal nematodes of domesticated chickens and the cause of avian ascaridiasis, a globally important disease in poultry. Its genome is sparsely sequenced and its proteome is uncharacterized. We her investigate the proteome composition in larvae and in female and male worms of A. Galli, querying the LC-MS/MS data against a hybrid database of two related nematodes; Ascaris suum and Anisakis simplex. We compare the proteome of larvae, female and male worms.
Project description:The bryophyte was collected and dried. Aqueous extract was prepared and different concentration of the extracts were treat against Ascardia galli, chicken nematode and Trichostrongylus, sheep nematode. Control was maintained for both. The control and treated samples were further processed to study the gene expression by isolating and sequencing mRNA using Illumina next-gen sequencing.
Project description:Co-infections with nematodes and protozoa occur frequently in free-ranging chickens and lead to complex local and systemic physiological responses, particularly in immune and metabolic pathways. This study aimed to investigate tissue-specific transcriptomic regulation in chickens co-infected with Ascaridia galli and Heterakis gallinarum, later developing a Histomonas meleagridis infection. The study focused on male birds of three chicken strains with different growth rates i.e. Lohmann Brown-LB, Lohmann Dual-LD, and Ross-308 and on three tissues; jejunum, caecum, and liver as these organs are predilection sites for the parasite life cycle, and are central in host nutrient absorption, local and systemic immune responses, and metabolic regulation.
