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PROVIDER: PRJEB21954 | ENA |
REPOSITORIES: ENA
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Project description:This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata. Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp). This submission includes raw sequencing data (FASTQ files) for all samples.
2026-01-13 | E-MTAB-16409 | biostudies-arrayexpress
Project description:Soybean (Glycine max) and mung bean (Vigna radiata) are key legumes with global importance, but their mechanisms for coping with cold stress—a major challenge in agriculture—have not been thoroughly investigated, especially in a comparative study. This research aimed to fill this gap by examining how these two major legumes respond differently to cold stress and exploring the role of uniconazole, a potential stress mitigator. Our comprehensive approach involved transcriptomic and metabolomic analyses, revealing distinct responses between soybean and mung bean under cold stress conditions. Notably, uniconazole was found to significantly enhance cold tolerance in mung bean by upregulating genes associated with photosynthesis, while its impact on soybean was either negligible or adverse. To further understand the molecular interactions, we utilized advanced machine learning algorithms for protein structure prediction, focusing on photosynthetic pathways. This enabled us to identify LOC106780309 as a direct binding target for uniconazole, confirmed through isothermal titration calorimetry. This research establishes a new comparative approach to explore how soybean and mung bean adapt to cold stress, offers key insights to improve the hardiness of legumes against environmental challenges, and contributes to sustainable agricultural practices and food security.
2024-07-19 | GSE193328 | GEO
Project description:Salinity is a major environmental constraint limiting crop productivity. Chromatin accessibility plays a crucial role in transcriptional regulation under stress conditions. In this study, we investigated genome-wide chromatin accessibility dynamics in mung bean (Vigna radiata cv. Weilv 9) leaves under salt stress using ATAC-seq. Two treatment groups were established: T1 (pre-conditioned with 100 mM NaCl for 24 h, recovered for 48 h, then re-exposed to 100 mM NaCl) and T2 (direct exposure to 100 mM NaCl without preconditioning). Seedlings were grown hydroponically in half-strength Murashige and Skoog medium under controlled environmental conditions. Nuclei were isolated from the first fully expanded true leaves at 0, 6, and 48 h after the onset of the final salt treatment, with three biological replicates per condition. Comparative ATAC-seq analysis revealed dynamic changes in chromatin accessibility associated with transcriptional reprogramming and stress memory. This dataset provides valuable insights into the epigenetic basis of salt tolerance and acclimation in legumes.
2026-06-07 | GSE334478 | GEO
Project description:DNA methylation is an important epigenetic mechanism regulating gene expression and stress memory in plants. To investigate the role of DNA methylation in salt stress adaptation, we performed whole-genome bisulfite sequencing (WGBS) of mung bean (Vigna radiata cv. Weilv 9) leaves under two treatment regimes: T1 (pre-conditioned with 100 mM NaCl for 24 h, recovered for 48 h, then re-exposed to 100 mM NaCl) and T2 (direct exposure to 100 mM NaCl without preconditioning). Seedlings were grown hydroponically in half-strength Murashige and Skoog medium under controlled environmental conditions. Genomic DNA was extracted from the first fully expanded true leaves at 0, 6, and 48 h after the onset of the final salt treatment, with three biological replicates per condition. Bisulfite-converted DNA was sequenced to generate genome-wide methylation profiles. Comparative analysis revealed dynamic changes in CG, CHG, and CHH contexts, providing insights into the epigenetic basis of salt tolerance and stress priming in legumes.
2026-06-07 | GSE334479 | GEO
Project description:Fungi associated with PBSA and Mung Bean (Vigna radiata L.) Raw sequence reads
| PRJNA818504 | ENA
Project description:<p> The pathophysiology of alcoholic-associated liver disease (ALD) is multifaceted. Utilizing the gut-liver axis framework and integrated multi-omics approaches, this study systematically evaluated the therapeutic potential of mung bean (Vigna radiata L.) ethanol extracts (MBE) in ALD, and its regulatory effects on gut microbiota and serum metabolites. Chemical analysis identified vitexin, isovitexin, catechin, trigonelline, and caffeic acid as MBE’s primary bioactive compounds. In a modified NIAAA model, MBE alleviated liver injury, lipid dysregulation, inflammation, oxidative stress, and gut barrier damage via PPARα-mediated lipid metabolism and Nrf2-driven antioxidant activation. 16S rRNA sequencing and in vitro fermentation experiment revealed that MBE specifically enriched Lactobacillus johnsonii in vivo and promoted its growth in vitro, with this bacterium being closely associated with increased spermidine levels. Lactobacillus johnsonii supplementation replicated MBE’s hepatoprotection by increasing spermidine and mitigating alcohol-induced hepatic/intestinal damage. Subsequent in vivo and in vitro spermidine interventions further validated its hepatoprotection. These findings propose a novel “MBE-Lactobacillus johnsonii-spermidine-liver” regulatory mode, positioning MBE as a dietary or therapeutic candidate for ALD and offering gut-liver axis targets.</p>
2025-10-10 | MTBLS13109 | MetaboLights
Project description:Mung bean (Vigna radiate) sprouts are a popular choice among sprouted vegetables in Asia. Currently, the impact of nitrogen sources on the growth of mung bean sprouts remains poorly understood, and the underlying biological mechanisms responsible for the observed nonlinear growth patterns at different nitrogen levels have yet to be elucidated. In this research, in addition to conventional growth monitoring and quality evaluation, a comparative proteomics method was applied to investigate the molecular mechanisms of mung bean in response to 0, 0.025, 0.05, 0.075, and 0.1% urea concentrations.Our results indicated that mung bean sprout height and yield increased with rising urea concentrations but were suppressed beyond theL3 level (0.075% urea). Nitrate nitrogen and free amino acid content rose steadily with urea levels, whereas protein content, nitrate reductase activity, and nitrite levels followed apeak-then-decline trend, peaking at intermediate concentrations. Differential expression protein analysis was conducted on mung bean sprouts treated with different concentrations of urea, and more differentially expressed proteins participated in the L3 urea concentration. Analysis of common differential proteins among comparison groups showed that the mung bean sprouts enhanced their adaptability to urea stress environments by upregulating chlorophyll a-b binding protein and cationic amino acid transporter and downregulating the levels of glycosyltransferase, L-ascorbic acid, and cytochrome P450.Theproteomic analysis uncovered the regulatory mechanisms governing these metabolic pathways, identifying 47 differentially expressed proteins (DEPs) involved in the biosynthesis of proteins, free amino acids, and nitrogen-related metabolites.
| PXD066373 |
Project description:RNA-seq analysis of multiple tissues in wild mung bean (Vigna radiata var. sublobata)
| PRJEB105588 | ENA
Project description:The immunomodulatory effect of mung bean is mainly attributed to antioxidant properties of flavonoids; however, the precise machinery for biological effect on animal cells remains uncertain. The objective of this study was to understand the physiological change produced by mung bean consumption.
2017-04-06 | PXD005330 | Pride