Project description:We sampled the microbial community at the sea ice edge in McMurdo Sound, Ross Sea at the same location (-77.62S, 165.41E) for four weeks (as described in Wu et al 2019, Nat. Comms.). We had four sampling dates corresponding to weeks 1 to 4: December 28 2014, January 6, 15, and 22 2015. Large volumes of water (150--250 L) were filtered from 1 m depth at the sea ice edge, and passed through three filters sequentially (3.0, 0.8, and 0.1 um, each 293 mm Supor filters). Filters with collected biomass were then placed in tubes with a sucrose-based preservative buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris-HCl, pH 8.0) and stored at -80 C until sample processing. We extracted proteins after buffer exchange into a 3\% SDS solution as previously described Wu et al 2019, Nat. Comms.
Project description:Samples were collected daily (19.06.2023-10.07.2023) the in the Waste water treatment plant "Rieselfelder" in Muenster, Germany, as full-day average sample. During the collection period, a festival took place in Muenster (24.06.2023). Field blanks of miliq were processed like the samples.
250 mL of sample were filtered 0.2 um cellulose acetate filters and extracted using Agilent Bond Elut PPL cartridges. Elution was performed with MeOH. Samples were reduced to dryness under an N2 stream and dissolved in 1 mL (MeOH:H2O (1:1, v/v)). UHPLC was performed using a Waters Acquity BEH C18 column (150 mm length, 1.7 um pore size). Eluents were miliq water (0.1% FA) and Acetonitrile (0.1% FA).
Data was acquired on a timsTOF fleX in PASEF mode in positive and negative polarity and an Agilent 6530A qTOF in positive and negative polarity.
Project description:This project includes water samples that were collected in West Maui, Hawaii, USA following the August 2023 wildfires. The majority of samples were collected along the shoreline, both inside and outside of the burn zone. A subset of samples were collected along an offshore transect. Another subset of samples were collected in or at the mouth of streams within the burn zone. All water samples consisted of 200 ml of water, pre-filtered with 0.2 um sterivex, run over Agilent HLB columns, and eluted with methanol.
2024-12-17 | MSV000096680 | MassIVE
Project description:Microbial community of 5 um filtered lake water
Project description:Analysis of bacterial fraction collected on GF/F filters post pre-filtration on 1um filter. 15L were filtered from Bering Strait (BSt) surface water and Chukchi Sea (station 2) bottom waters.
Project description:To clarify the effects of near-infrared radiation, we assessed DNA microarray after water-filtered near-infrared (1100-1800 nm together with a water-filter that excludes wavelengths 1400-1500 nm) irradiation.
Project description:In February 2025, flowing river water was sampled from the banks of the Ruhr, Rhine, Emscher and Lippe rivers in Germany. On the same day, a purified water blank was taken and stored over the same time period. All samples were immediately cooled after sampling, frozen as soon as possible, and stored at -20 C. After thawing, 250 mL were filtered with 0.2 um cellulose acetate syringe filters and extracted by Agilent Bond Elute LMS cartridges. Elution was performed with MeOH. Samples were reduced to dryness under an N2 stream and dissolved in 1 mL (MeOH:H2O (1:1, v/v)). UHPLC was performed using a Waters Acquity BEH C18 column (150 mm length, 1.7 um pore size). Data were acquired on a timsTOF fleX in PASEF mode in positive and negative polarity. Eluents were purified water (0.1% FA) and acetonitrile (0.1% FA). Data were also acquired on a 6530 Q-TOF LC/MS in DDA mode in positive and negative polarity. For this, eluents were purified water and acetonitrile.
Project description:Effluent from geoduck clam larval rearing tanks at two different pH (8.2 and 7.1) was collected at 4 time points (Days 1, 5, 8, and 12) over 12 days in a shellfish hatchery in Washington state, USA. The water was filtered to 0.2 microns to retain the bacterial fraction.