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RNA-Seq of perirenal adipose tissue of Assaf suckling lambs

ABSTRACT: RNA-Seq of perirenal adipose tissue of Assaf suckling lambs

PROVIDER: PRJEB53616 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR9853480_1.fastq.gz	Fastqsanger.gz
	ERR9853480_2.fastq.gz	Fastqsanger.gz
	ERR9853481_1.fastq.gz	Fastqsanger.gz
	ERR9853481_2.fastq.gz	Fastqsanger.gz
	ERR9853482_1.fastq.gz	Fastqsanger.gz

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RNA-Seq of perirenal adipose tissue of Assaf suckling lambs

Project description:Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were selected from a larger group of 17 Assaf suckling lambs for which carcass traits were measured. The 8 selected lambs were those showing the highest and the lowest values, from the larger group, for the percentage of perirenal and cavitary fat relative to the half carcass weight. Hence, considering the values for this trait, we defined the High-PF group (n = 4; average: 3.23 ± 0,.47) and the Low-PF group (n = 4; 1.65 ± 0,.16), respectively.

2023-04-25 | E-MTAB-11839 | biostudies-arrayexpress

RNA-seq of perirenal fat from male and female Assaf suckling lambs

Project description:In sheep, differences were observed regarding fat accumulation and fatty acid composition between males and females, which may impact the quality and organoleptic characteristics of the meat. The analysis of omics technologies is a relevant approach for investigating biological and genetic mechanisms associated with complex traits. Here, the perirenal tissue of six male and six female Assaf suckling lambs was evaluated using RNA sequencing.

2022-10-12 | E-MTAB-12130 | biostudies-arrayexpress

Suckling lamb perirenal fat transcriptome

Project description:The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf= 8, Churra= 6) was used for the RNA-Seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty-acid oxidation, while in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, while the Churra upregulated DEGs are linked to fat accumulation.

2018-07-18 | GSE108314 | GEO

RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age

Project description:Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were offspring of dams subjected to a Nutritional Protein Restriction (NPR) at the prepubertal age as described by Mateo et al. (2023) [doi: 10.1016/j.livsci.2023.105173]. These animals were reared in the same batch that other 8 Assaf suckling lambs obtained from Control dams of the NPR experiment for which RNA-Seq samples are already available (see E-MTAB-11839 for RNA-Seq data of Control lambs). These 8 NPR animals were selected from a larger group of 17 NPR suckling lambs for which carcass traits were measured. The 8 selected lambs were those from the 17 initially considered lambs showing the highest and the lowest values , from the larger group, for the percentage of kidney knob and channel fat relative to the right half carcass weight (KKCF). Hence, considering the values for this trait, we defined the High-KKCF group (n = 4; average: 3.26 ± 0.11) and the Low-KKCF group (n = 4; 1.68 ± 0.25), respectively.

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RNA-seq of perirenal fat from male and female Assaf suckling lambs

Project description:RNA-seq of perirenal fat from male and female Assaf suckling lambs

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Whole-Genome Bisulfite Sequencing analysis of perirenal fat of Assaf suckling lambs from ewes subjected to nutritional challenge and control ewes

Project description:Whole-Genome Bisulfite Sequencing analysis of perirenal fat of Assaf suckling lambs

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RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age

Project description:RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age

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Suckling lamb perirenal fat transcriptome

Project description:Suckling lamb perirenal fat transcriptome

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Project description:Because of the central role of the liver in numerous physiological processes and its possible relation with quality carcass traits in suckling lambs, and considering that female lambs have higher carcass fatness levels, this experiment has analyzed the liver transcriptome of 12 suckling lambs (slaughter age: +/- 25 days), including six males and six females. RNA was extracted from the liver tissue of the lambs at the time of slaughter following a standard protocol. The RNA integrity value was calculated using the Agilent 2,100 Bioanalyzer equipment (Agilent Technologies, CA, USA). cDNA libraries were sequenced to a minimum depth of 30 million paired-end reads, generating 150 bp stranded paired-end reads using an Illumina Novaseq 6,000 system.

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