Project description:This study aimed to characterize and compare the transcriptomic profiles of perirenal and tail adipose tissues in semi-tailed Assaf lambs of approximately one month of age. 16 paired RNA-Seq samples from perirenal and tail fat of eight lambs were obtained.
Project description:Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were selected from a larger group of 17 Assaf suckling lambs for which carcass traits were measured. The 8 selected lambs were those showing the highest and the lowest values, from the larger group, for the percentage of perirenal and cavitary fat relative to the half carcass weight. Hence, considering the values for this trait, we defined the High-PF group (n = 4; average: 3.23 ± 0,.47) and the Low-PF group (n = 4; 1.65 ± 0,.16), respectively.
Project description:In sheep, differences were observed regarding fat accumulation and fatty acid composition between males and females, which may impact the quality and organoleptic characteristics of the meat. The analysis of omics technologies is a relevant approach for investigating biological and genetic mechanisms associated with complex traits. Here, the perirenal tissue of six male and six female Assaf suckling lambs was evaluated using RNA sequencing.
Project description:The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf= 8, Churra= 6) was used for the RNA-Seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty-acid oxidation, while in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, while the Churra upregulated DEGs are linked to fat accumulation.
Project description:Samples of perirenal fat tissue from 8 Assaf breed suckling lambs. These animals were offspring of dams subjected to a Nutritional Protein Restriction (NPR) at the prepubertal age as described by Mateo et al. (2023) [doi: 10.1016/j.livsci.2023.105173]. These animals were reared in the same batch that other 8 Assaf suckling lambs obtained from Control dams of the NPR experiment for which RNA-Seq samples are already available (see E-MTAB-11839 for RNA-Seq data of Control lambs). These 8 NPR animals were selected from a larger group of 17 NPR suckling lambs for which carcass traits were measured. The 8 selected lambs were those from the 17 initially considered lambs showing the highest and the lowest values , from the larger group, for the percentage of kidney knob and channel fat relative to the right half carcass weight (KKCF). Hence, considering the values for this trait, we defined the High-KKCF group (n = 4; average: 3.26 ± 0.11) and the Low-KKCF group (n = 4; 1.68 ± 0.25), respectively.
Project description:RNA-Seq of perirenal adipose tissue of Assaf suckling lambs whose dam were subjected to a dietary protein restriction at prepubertal age