Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| variant-BMPR1B.vcf.csi | Other | |||
| variant-BMPR1B.vcf.gz | Vcf |
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Project description:Background: The anterior pituitary plays a crucial role in endocrine regulation and homeostasis by orchestrating hormonal signaling, cellular growth, and functional adaptations. Adipokines regulate hypothalamic-pituitary-ovarian axis influencing hormone secretion and cellular processes. Omentin-1, an adipokine primarily expressed in visceral adipose tissue, is involved in metabolic and reproductive functions. However, its role in pituitary remains unknown. We hypothesize that omentin-1 modulates protein abundance in pituitary cells. This study aimed to investigate role of omentin-1 in regulating key pathways in anterior pituitary cells of pigs with different metabolic statuses: normal-weight Large White pigs and genetically obese Meishan pigs. Results: High-throughput proteomic analysis of porcine anterior pituitary cells exposed to omentin-1 revealed 230 differentially abundant proteins (30 downregulated and 3 upregulated in Large White pigs, as well as 107 downregulated and 90 upregulated in Meishan pigs), associated with 116 gene ontology terms. The most significantly altered proteins were associated with cell cycle and DNA replication, gene expression regulation and posttranscriptional/posttranslational modifications. Apoptotic and proliferative mechanisms varied between breeds, suggesting different metabolic adaptation strategies. In Large White pigs, CDK5RAP2 and SIX1 played a key role in transcriptional and proliferative regulation, whereas in Meishan pigs, stronger proliferative control was linked to AKT1S1 and RHOA. Moreover, Meishan pigs demonstrated a more dynamic regulation of apoptotic pathways (HTRA2, PARP2, DFFA), which may promote greater metabolic plasticity and better adaptation to physiological fluctuations. Additional in vitro experiments in Large White pigs showed that omentin-1 influenced key proliferative and apoptotic markers, downregulating cyclins D1, E1, A2, and B1, as well as caspase-3, while upregulating the anti-apoptotic protein BCL2, leading to an increased BCL2/BAX ratio. Further, omentin-1 modulated phosphorylation of selected kinases, including ERK1/2, AKT, AMPKα, and STAT3, in a time-dependent manner. It is also increased insulin receptor expression and promoted packaging and transport of secretory proteins. Conclusions: Proteomic and functional analyses revealed that omentin-1 regardless of breed exerts anti-proliferative and anti-apoptotic effects on porcine anterior pituitary cells, modulating key signaling pathways. These findings highlight that omentin-1 is a potential metabolic regulator influencing anterior pituitary function.
2025-06-17 | PXD061269 | Pride
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs.
2009-06-20 | GSE11853 | GEO
Project description:Backfat thickness is one of the most important traits of commercially raised pigs. Meishan pigs are renowned for having thicker backfat than Landrace pigs. To examine the genetic factors responsible for the differences, we first produced female crossbred pig lines by mating Landrace (L) × Large White (W) × Duroc (D) females (LWD) with Landrace (L) or Meishan (M) boars (i.e., LWD × L = LWDL for Landrace offspring and LWD × M = LWDM of the Meishan offspring). We confirmed that LWDM pigs indeed had a thicker backfat than LWDL pigs. Next, we performed gene expression microarray analysis (in both genetic lines) to examine differentially expressed genes (DEGs) in energy metabolism-related tissues, subcutaneous adipose (fat), liver, and longissimus dorsi muscle tissues. We analyzed the annotation of DEGs (2-fold cutoff) to functionally categorize them by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. The number of DEGs in muscle tissues of both lines was much less than that in fat and liver tissues, indicating that DEGs was much lesser in muscle tissues (in both genetic lines) than in fat and liver tissues, thus indicating that DEGs in muscle tissues may not contribute much to differences in backfat thickness. In contrast, several genes related to muscle (in fat tissue) and fatty acid and glucose metabolism (in the liver) were more upregulated in LWDM pigs than LWDL pigs, indicating that those DEGs might be responsible for differences in backfat thickness. The different genome-wide, gene expression profiles in the fat, liver, and muscle tissues between breeds can provide useful information for pig breeders.
2018-09-06 | GSE97711 | GEO
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
2008-01-07 | GSE8377 | GEO
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs. Experiment Overall Design: Placenta efficiency (the body weight of a piglet divided by the mass of its placenta) of Erhualian pigs is markedly higher than Large White pigs. so placenta samples (female) from 6 Erhuanlian pregnant gilts at gestation day 75 (E75) and day 90 (E90) and 6 Large White pregnant gilts at gestation day 75 (L75) and day 90 (L90) were collected. RNAs from two female piglet placentas from each gilt were combined to 12 pools and hybridized to the porcine Affymetrix GeneChip.
2009-07-01 | E-GEOD-11853 | biostudies-arrayexpress
Project description:We collected the ovary samples at 49 days post coitus in Large White and Meishan adult female pigs, identified the differentially expressed protein coding genes and long non-coding RNAs between these two breeds. three individuals of each breed were harvested as biological replicates.
2017-06-12 | GSE73593 | GEO
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples
2010-06-29 | E-GEOD-8377 | biostudies-arrayexpress
Project description:Affymetrix miRNA arrays were used to generate miRNA profiles in placenta of Meishan and Yorkshire pigs on stages of initiation (gestational day 26) and establishment (gestational day 50) of placental folds development. The study allowed for the determination of the miRNAs that were differentially expressed in porcine placenta on different gestational day. This study will provide the information to better understanding of the role miRNAs in porcine placental development.
2015-10-01 | GSE62996 | GEO
Project description:Numerous circular RNAs (circRNAs) have been recently identified in porcine tissues and cell types. Nevertheless, their significance in porcine spleen development is yet unelucidated. Herein, we report an extensive overlook of circRNA expression profile during Meishan pig spleen development at 1, 7, 14, 21, 28, 35, 120, and 180 days of age. Overall, 39,641 circRNAs were identified from 6,914 host genes. Among them, many circRNAs are up- or down-regulated at different time points of pig spleen development. Using WGCNA analysis, we revealed two essential modules for protein-coding genes and circRNAs. Subsequent correlation analysis revealed 67 circRNAs/co-expressed genes that participated in immnue-associated networks. Furthermore, a competing endogenous RNA (ceRNA) network analysis of circRNAs revealed that 12 circRNAs modulated CD226, MBD2, SAMD3, SIT1, SRP14, SYTL3 gene expressions via acting as miRNA sponges. Moreover, the circRNA_21767/miR-202-3p axis regulated SIT1 expression in a ceRNA manner, which is critical for the immune-based regulation of spleen development in Meishan pigs.
2023-04-11 | GSE228936 | GEO