Project description:NIH-3T3 cells were infected with wildtype MCMV at a multiplicity of infection (MOI) of 10. Ribosome profiling was performed at the indicated times of infection as described in Rutkowski et al., Nature Communications 2015.
Project description:We asked how knockdown of genes associated with neurodegeneration using antisense oligonucleotides (ASO) affects disease phenotypes in an inducible TDP-43 transgenic mouse model (rNLS8, Walker et al., Acta Neuropathologica 2015) backcrossed to the C57BL/6 background. This dataset includes only the non-targeting control, which was also used as a replication cohort for transcriptional changes in rNLS8 mice (Rezaei et al., in revision at Nature Communications). We show cholesterol dysmetabolism and marker genes of disease-associated oligodendrocytes in the brain of rNLS8 mice. Data from mice receiving the targeting ASOs will be made available upon publication of the manuscript (Kocsis-Jutka et al, in preparation).
Project description:Key regulators of septum formation between the left and right ventricle in mammals, including the transcription factors TXB5 and PITX2, feature loss-of-function phenotypes that affect development of the two-chambered zebrafish heart, suggesting uncharacterized primordial functions of these septation regulators prior to the evolution of any physical boundaries between heart chambers. Data is as published in Mosimann & Panakova et al (Nature Communications, 2015)
Project description:Fastq files for the 16S rDNA amplicon library of 714 fecal samples of 20 time series (as described in Vandeputte et al. 2021, Nature Communications)
Project description:Lineage tracing of individual cells during directed differentiation human iPSC into alveolospheres was performed using a lentiviral barcode labeling system (Weinreb et al., 2020) as described in Hurley et al., 2020.
Project description:The LM2 derivative cell line is described in Minn et al. Nature 2005. The LM1a derivative cell line is described in Tavazoie et al. Nature 2008.
Project description:drl expression initiates during gastrulation and condenses as a band of cells at the prospective lateral embryo margin. In late epiboly, drl:EGFP is detectable as a band of scattered EGFP-fluorescent cells; after gastrulation, drl:EGFP-positive cells coalesce at the embryo margin that then in somitogenesis break down into the anterior and posterior lateral plate with subsequent cell migrations that form the posterior vascular/hematopoietic stripes and the anterior cardiovascular and myeloid precursors. Data is as published in Mosimann & Panakova et al (Nature Communications, 2015)
Project description:Key regulators of septum formation between the left and right ventricle in mammals, including the transcription factors TXB5 and PITX2, feature loss-of-function phenotypes that affect development of the two-chambered zebrafish heart, suggesting uncharacterized primordial functions of these septation regulators prior to the evolution of any physical boundaries between heart chambers. Data is as published in Mosimann & Panakova et al (Nature Communications, 2015) Samples for tbx5a versus pitx2ab MO comparison were collected from three independent experiments of MO-injected siblings and uninjected wildtype controls at 56hpf by morphology.