Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HKU1 isolate in primary cells

ABSTRACT: HKU1 isolate in primary cells

PROVIDER: PRJEB64017 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR11642244.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Superficieibacter sp. HKU1 isolate:hospitalised patient

Project description:Superficieibacter sp. HKU1 isolate:hospitalised patient Genome sequencing

| PRJNA940906 | ENA

HKU1 internal protein ameliorate infection in mice

Project description:Coronaviruses express a repertoire of accessory proteins for evading host immune responses. Among these accessory proteins, a small internal (I) protein is expressed by members of the genus Betacoronavirus. Previous studies reported that the I proteins of SARS-CoV, MERS-CoV and SARS-CoV-2 inhibit type I interferon (IFN-I) expression through distinct mechanisms and play different roles in pathogenesis. In addition, human coronaviruses HKU1 and OC43 are betacroaonvairuses that predominantly cause common cold and encode the I protein as one of their accessory proteins; the I proteins of hCoV-HKU1 and hCoV-OC43 have not been previously characterized. However, the lack of robust reverse genetic systems, tissue culture and animal models limit the study of hCoV-HKU1 and hCoV-OC43 pathogenesis. Here, we examined the role of hCoV-HKU1 and hCoV-OC43 I proteins in pathogenesis using a prototypic coronavirus. We introduced the I proteins of hCoV-HKU1 and hCoV-OC43 independently to a neurotropic strain of mouse hepatitis virus (MHV-J2.2). MHV-J2.2 infection is well-characterized with clearly defined immune responses which allows the study of I proteins in the context of authentic coronavirus infection. We showed that the I protein of hCoV-HKU1 but not that of hCoV-OC43 ameliorated MHV-J2.2 infection while the I protein of MERS-CoV causes exacerbated disease. Further analysis revealed that infection with MHV-J2.2 expressing the I protein of MERS-CoV leads to increased neutrophil infiltration to the site of infection and virus titers in mice; diminished virus titers was observed in the presence of hCoV-HKU1 I protein. Overall, our findings suggest that the I protein of different betacoronaviruses play unique roles in pathogenesis.

2025-04-23 | GSE291309 | GEO

Staphylococcus sp. HKU1

Project description:Staphylococcus sp. HKU1 Genome sequencing

| PRJNA1004220 | ENA

Deciphering the O-glycosylation of HKU1 Spike Protein with the Dual-Functional Hydrophilic Interaction Chromatography Materials

Project description:HKU1 is a human betacoronavirus and infects host cells via highly glycosylated spike protein (S). At present, N-glycosylation of HKU1 S has been reported, however, little is known about its O-glycosylation, which hinders an in-depth understanding of its biological functions. Herein, a comprehensive study of O-glycosylation of HKU1 S was carried out based on dual-functional histidine-bonded silica materials (HBS). The enrichment method for O-glycopeptides with HBS was developed and validated using standard proteins. The application of the developed method to HKU1 S1 subunit resulted in 61 novel O-glycosylation sites, among which 56% were predicted to be exposed on protein outer surface. Moreover, the O-linked glycans and their abundance on each HKU1 S1 site were also analyzed. The obtained O-glycosylation dataset would provide valuable insights for the understanding of the structure of HKU1 S.

2022-02-17 | PXD025967 | Pride

Streptococcus pneumoniae strain:HKU1-14

Project description:Streptococcus pneumoniae strain:HKU1-14 Genome sequencing and assembly

| PRJNA361421 | ENA

HKU1 internal protein ameliorate infection in mice

Project description:HKU1 internal protein ameliorate infection in mice

| PRJNA1232806 | ENA

POWV isolate from Long Island infects brain endothelial cells and pericytes. [hBVPs]

Project description:POWV isolate from Long Island (POWV-LI-9) is released basolaterally from humban brain microvascular endothelial cells (hBMECs) and infects primary human brain vascular pericutes (hBVPs).

2021-06-08 | GSE176252 | GEO

POWV isolate from Long Island infects brain endothelial cells and pericytes. [hBMECs]

2021-06-08 | GSE176251 | GEO

Gallus gallus isolate:Primary culture chicken myocardial cells

Project description:Gallus gallus isolate:Primary culture chicken myocardial cells Raw sequence reads

| PRJNA394944 | ENA

Homo sapiens isolate:Primary

Project description:Homo sapiens isolate:Primary Raw sequence reads

| PRJNA1080505 | ENA