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Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

ABSTRACT: Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

PROVIDER: PRJEB70872 | ENA |

REPOSITORIES: ENA

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Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

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Whole Genome Long Read Sequencing of splenocytes from Four Core Genotype (FCG) mice

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Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains single-cell gene expression profiling from mouse splenocytes of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3 , four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. From all four genotypes, splenocytes are isolated and subjected to 10x Genomics single-cell RNA-Sequencing.

2024-10-14 | E-MTAB-13586 | biostudies-arrayexpress

Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

Project description:Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

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Whole Genome Long Read Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains whole genome long read sequencing data generated using Oxford Nanopore Technologies from a mouse of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. DNA sequencing was done on a liver sample of the XYSry+ genotype.

2024-10-14 | E-MTAB-14291 | biostudies-arrayexpress

Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains whole genome sequencing data from mice of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3 , four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes, or on a pure-bred C57BL6 background. DNA sequencing was done on either liver or ear-punch samples.

2024-10-14 | E-MTAB-13585 | biostudies-arrayexpress

Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

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Liver tissue from the "Four Core Genotype" treated group C57BL/6J mice versus pool C57BL/6J mice of chow diet Liver

Project description:The purpose of this experiment was to determine the expression traits in Liver tissue from the Four Core Genotype treated group. Keywords: Sry transgene Four Core Genotype Mouse liver Tissue Liver tissue from the "Four Core Genotype" treated group consists of 20 female and male C57BL/6J mice fed a chow diet containing 4% fat (Ralston-Purina Co., St. Louis, MO) until 8 weeks of age and then were gonadectomized at 8 weeks of life. In mice of the "four core genotypes" (FCG), the Y chromosome is deleted for the testis-determining gene Sry, producing the Y- chromosome. The Sry transgene is inserted onto an autosome, so that testis determination is independent of the complement of sex chromosomes. XY-Sry gonadal males are bred with XX gonadal females, producing progeny with four different genotypes: two types of gonadal males (XX.Sry and XY-Sry) and two types of gonadal females (XX and XY-). At 12 weeks mice were sacrificed, after a 12-hour fast, Liver tissue were dissected and flash frozen in LN2 and stored at -80Â°C. All sample were compared to a common pool created from equal portions of RNA from each of the samples.

2010-06-24 | E-GEOD-13264 | biostudies-arrayexpress

Liver tissue from the "Four Core Genotype" treated group C57BL/6J mice versus pool C57BL/6J mice of chow diet Liver

2008-12-08 | GSE13264 | GEO

Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

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2024-10-15 | E-MTAB-13700 | biostudies-arrayexpress

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