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Whole Genome Long Read Sequencing of splenocytes from Four Core Genotype (FCG) mice

ABSTRACT: Whole Genome Long Read Sequencing of splenocytes from Four Core Genotype (FCG) mice

PROVIDER: PRJEB78377 | ENA |

REPOSITORIES: ENA

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Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

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Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

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Whole Genome Long Read Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains whole genome long read sequencing data generated using Oxford Nanopore Technologies from a mouse of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. DNA sequencing was done on a liver sample of the XYSry+ genotype.

2024-10-14 | E-MTAB-14291 | biostudies-arrayexpress

Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains single-cell gene expression profiling from mouse splenocytes of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3 , four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. From all four genotypes, splenocytes are isolated and subjected to 10x Genomics single-cell RNA-Sequencing.

2024-10-14 | E-MTAB-13586 | biostudies-arrayexpress

Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

Project description:Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

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Whole Genome Sequencing of splenocytes from Four Core Genotype (FCG) mice

Project description:This repository contains whole genome sequencing data from mice of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3 , four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes, or on a pure-bred C57BL6 background. DNA sequencing was done on either liver or ear-punch samples.

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Single-nucleus RNA-Sequencing (sci-RNA-seq3) of whole livers from 48 Four Core Genotype (FCG) mice

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Liver tissue from the "Four Core Genotype" treated group C57BL/6J mice versus pool C57BL/6J mice of chow diet Liver

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2010-06-24 | E-GEOD-13264 | biostudies-arrayexpress

Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

Project description:This repository contains single-nucleus gene expression profiling from mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes are on a C57BL6 genetic background. From all four genotypes, whole livers were dissected and flash frozen, followed by nuclear extraction and fixation for sci-RNA-seq3.

2024-10-15 | E-MTAB-13700 | biostudies-arrayexpress

Liver tissue from the "Four Core Genotype" treated group C57BL/6J mice versus pool C57BL/6J mice of chow diet Liver

2008-12-08 | GSE13264 | GEO