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Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

ABSTRACT: Single-nucleus RNA-Sequencing of whole livers from Four Core Genotype (FCG) mice

PROVIDER: PRJEB75026 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	ERR12916521_1.fastq.gz	Fastqsanger.gz
	ERR12916521_2.fastq.gz	Fastqsanger.gz
	ERR12916522_1.fastq.gz	Fastqsanger.gz
	ERR12916522_2.fastq.gz	Fastqsanger.gz
	ERR12916523_1.fastq.gz	Fastqsanger.gz

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Project description:This repository contains single-nucleus gene expression profiling from mouse livers of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3, four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes are on a C57BL6 genetic background. From all four genotypes, whole livers were dissected and flash frozen, followed by nuclear extraction and fixation for sci-RNA-seq3.

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