Project description:The aim of this study was to analyze if transcriptionally active svb loci show enrichment for H3K4me1. To capture cells where svb-related ventral enhancers are active, we FACS-sorted nuclei from stage 15 Drosophila melanogaster embryos based on the expression of different reporter genes driven by different svb enhancers. The svb_E10 enhancer drives the expression of GFP, whilst dsRed is driven by the svb_7 enhancer. The sorted nuclei were then used for ChIP with anti-H3K4me1 and anti-H3 (for normalization purposes) antibodies.

2022-12-12 | E-MTAB-12396 | biostudies-arrayexpress

Synonymous codon massive reporter library in zebrafish embryos

Project description:Synonymous codon massive reporter library in zebrafish embryos

| PRJNA856272 | ENA

Highly parallel assays of tissue-specific enhancers in whole Drosophila embryos

Project description:Understanding transcriptional regulatory networks requires the identification and characterization of cis-regulatory modules (CRMs), DNA sequences which can direct expression of associated genes to a specific cell type and/or developmental stage. Reporter assays for the capacity of a candidate CRM to activate a heterologous promoter have been productive but suffer limited throughput or fail to convey information on cell type specificity. We have developed an assay in which reporter constructs containing a pool of candidate CRMs are introduced in parallel to Drosophila embryos along with a common cell-type-specific second marker; candidate CRMs isolated by PCR from FACS-purified double-positive cells can by quantitated by high-throughput sequencing, and their relative abundance compared to those in the input cell population to detect activity in the cell type (or types) of interest. twi-CD2+/GFP+ cells in triplicate, and input (twi-CD2+) cells in triplicate for comparison; twi-CD2-/GFP+ cells in triplicate, and input (twi-CD2-) cells in triplicate for comparison; mef2-CD2+/GFP+ cells (single sample), and input (mef2-CD2-) cells in quintuplicate for comparison and variance estimation; mef2-CD2-/GFP+ cells in triplicate, and input (mef2-CD2-) cells in triplicate for comparison; duf:CD2+/GFP+ cells (single sample), and input (duf:CD2-) cells in quintuplicate for comparison and variance estimation; duf:CD2-/GFP+ cells in sextuplicate, and input (duf:CD2-) cells in sextuplicate for comparison.

2013-07-13 | E-GEOD-41503 | biostudies-arrayexpress

H3K4me1 ChIP-Seq experiment using cells from stage 15 Drosophila melanogaster embryos sorted by reporter gene activity

Project description:H3K4me1 ChIP-Seq experiment using cells from stage 15 Drosophila melanogaster embryos sorted by reporter gene activity

| PRJEB57626 | ENA

Quantitative proteomes of Imp2 KO mouse 2-cell-stage embryos

Project description:Data showing the late 2-cell-stage, control embryos (Imp2♀+/♂+) and Imp2-knockout embryos (Imp2♀−/♂+) for HPLC MS/MS analysis. 3 replicates were performed using 330 embryos per group.

2019-11-12 | PXD010397 | Pride

Massively parallel reporter assays investigate shared genetic variants of eight psychiatric disorders

Project description:Massively parallel reporter assays investigate shared genetic variants of eight psychiatric disorders

| PRJNA1020890 | ENA

Embryos Assist Each Other’s Morphogenesis: calcium and ATP signaling mechanisms in collective resistance to teratogens (Stage 25)

Project description:Embryos Assist Each Other’s Morphogenesis: calcium and ATP signaling mechanisms in collective resistance to teratogens (Stage 25)

| PRJNA1029975 | ENA

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