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RNA-seq of A498 cells with deletion in HERVE 6q15

ABSTRACT: RNA-seq of A498 cells with deletion in HERVE 6q15

PROVIDER: PRJEB80889 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	ERR13764603_1.fastq.gz	Fastqsanger.gz
	ERR13764603_2.fastq.gz	Fastqsanger.gz
	ERR13764604_1.fastq.gz	Fastqsanger.gz
	ERR13764604_2.fastq.gz	Fastqsanger.gz
	ERR13764605_1.fastq.gz	Fastqsanger.gz

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RNA-seq of A498 cells with deletion in HERVE 6q15

Project description:HERVE 6q15 was deleted by CRISPR in A498 cells, which were then compared with parental A498 cells, with the aim of assessing the impact of this retroviral insertion

2025-04-18 | E-MTAB-14514 | biostudies-arrayexpress

Transcription profiling of human VHL treated A498 renal cancer cells

Project description:A498 cells, established from a clear cell renal cell cancer of a 52 years old male patient, were obtained from ATCC and maintained in DMEM supplemented with 10% fetal calf serum (FCS). This cell line does not express functional VHL. A498 subclones stably transfected with HA-pVHL30 or control plasmid have been described and were maintained in 0.5 mg/ml G418.

2003-09-18 | E-MEXP-13 | biostudies-arrayexpress

Transcriptome profiling of ANXA4 KO in A498 cells

Project description:Transcriptome profiling of ANXA4 KO in A498 cells

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Expression data profile of A498 cells treated with DRB or E. coli 83972

Project description:Urinary tract infections (UTIs) constitute a highly relevant model of microbial adaptation, in which the contrasting effects of pathogens and commensals on host tissues are clearly displayed. While virulent Escherichia coli cause severe, potentially life-threatening disease by breaking the inertia of the mucosal barrier and infecting the kidneys, the most common outcome of bacteriuria is an asymptomatic carrier state resembling commensalism at other mucosal sites. It remains unclear if the lack of destructive inflammation merely reflects low virulence or if carrier strains actively inhibit disease associated responses in the host. To address this question, we examined the effects of asymptomatic bacterial carriage on host gene expression. The asymptomatic strain E. coli 83972 caused reduction in Pol II phosphorylation in the nuclei of human kidney epithelial A498 cells. To specifically address if Pol II inhibition alters the response to infection, A498 cells were pretreated with 5,6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB). This adenosine analogue has been proposed to specifically and reversibly inhibit Pol II transcription without directly affecting other cellular functions. A498 cultered cells were infected with E. coli 83972 or DRB for 4 hours. The culture medium with DMSO was used as a background control. A498 cells were infected with E. coli 83972 or DRB for 4 h. Isolated RNA was subjected to whole genome transcriptome analysis.

2013-02-23 | E-GEOD-43886 | biostudies-arrayexpress

Transcriptome profiling of ANXA4 KO in A498 cells

Project description:ANXA4 WT (control) and ANXA4 KO in A498 kidney cancer cells was generated using CRISPR/Cas9 and two independent gRNAs for control and two independent gRNAs targeting ANXA4. Single cells clones (Ctrl.-1, Crtl.-2, KO-1, KO-2) were selected and validated by western blotting confirming loss of ANXA4 in KO cell lines on protein level. Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis of 2 independent replicates per cell line was performed (resulting in 4 control and 4 ANXA4 KO sequencings). ANXA4 KO cells exhibit transcriptional changes compared to WT cells including upregulation of the ETS transcription factor ELF3 and regulation of Matrisome genes.

2024-05-28 | GSE267754 | GEO

RNA-Seq Analysis

Project description:RNA-Seq analysis carried out in three different backcrosses based on Iberian breed with Landrace, Pietrain and Duroc breeds

2017-10-31 | PRJEB23068 | EVA

RNA-seq analysis of A498 cell line treated with siSETD2 or si-NC

Project description:RNA-seq analysis of A498 cell line treated with siSETD2 or si-NC

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Expression data profile of A498 cells treated with DRB or E. coli 83972

2013-02-23 | GSE43886 | GEO

HIF-2alpha Knockdown in A498 (VHL-/-) ccRCC cells

Project description:HIF-2alpha is essential for (VHL-/-) ccRCC subcutaneous tumor growth in mice, and in tumor cell lines, its inhibition results in increased ROS accumulation, tumor cell death and responsiveness to radiation treatment. We have utilized transcriptional profiling to screen for putative HIF-2alpha targets genes that serve an anti-oxidant and, thus, cell survival function. A498 ccRCC cell line was treated with control siRNA or mixture of two HIF-2alpha specific siRNA for 48 hours, and RNA was harvested. 4 independent experiments were performed, and expression was compared between control and HIF-2alpha knockdown groups.

2009-08-27 | GSE16622 | GEO

Exosome from A498 cellular supernatant

Project description:Exosome from A498 cellular supernatant

| PRJNA655690 | ENA