Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Small RNA seq of FACS sorted progenitor cells in Drosophila Intestine

ABSTRACT: Small RNA seq of FACS sorted progenitor cells in Drosophila Intestine

PROVIDER: PRJEB85278 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ERR14216200.fastq.gz	Fastqsanger.gz
	ERR14216201.fastq.gz	Fastqsanger.gz
	ERR14216202.fastq.gz	Fastqsanger.gz
	ERR14216203.fastq.gz	Fastqsanger.gz
	ERR14216204.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 9

Similar Datasets

Small RNA seq of FACS sorted progenitor cells in Drosophila Intestine

Project description:To gain a comprehensive view of the potential miRs involved in the regulation of the ISC lineage in the adult Drosophila midgut, this data represents the small RNA sequencing of isolated esg-positive cells. To maximize the opportunity of identifying ISC/EBs-specific specific miRs, around 50,000 GFP-positive cells were FACS sorted in triplicates for each genotype (controls and two Dcr-1 knock-downs).

2025-02-20 | E-MTAB-14781 | biostudies-arrayexpress

FACS sorted podocytes, mesangial cells

Project description:FACS sorted glomerular kidney cells from a single mouse were subjected to proteomics.

2022-10-13 | PXD016237 | Pride

Transcription profiling of Drosophila neurons visualized with GFP and FACS sorted

Project description:Expression profiling of Drosophila neurons visualized with transgenic reporter genes, 7.4 kb Cha-Gal:UAS-GFP or 2.1 kb Gad1(s)-RFP. Experiment Overall Design: Transgenic Drosophila embryo cultures (5-7 day old) incubated at 25 C, dissociated and harvested for Fluorescent Active Cell Sorting (FACS) prior to microarray analysis.

2009-04-04 | E-GEOD-10773 | biostudies-arrayexpress

Negative FACS sorted EC neurons

Project description:Drosophila Embryo Cell Culture samples allowed to differentiate 3-5 days in culture. Samples express a GFP reporter in cholinergic neurons (7.4 kb ChaGal4:UAS-GFP) or an RFP reporter (2.1 kb Gad1-RFP) in glutamic acid decarboxylase 1 expressing Neurons. This group of samples contains the negatively sorted FACS fractions (2 Cha and 2 Gad negative samples). Keywords: other

2004-07-04 | GSE1046 | GEO

FACS-sorted Six2-positive nephron progenitor cells at embryonic day 15.5: wildtype (WT) vs. conditional HDAC1&2-null (MUT)

Project description:Transcriptional profiling of FACS-sorted Six2-positive nephron progenitor cells from Six2CreEGFP mice without (WT) or with (MUT) homozygously floxed HDAC1 and HDAC2 alleles at the age of embryonic day 15.5. This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of HDAC1&2 in the nephron progenitor population and successive changes to the series of events in kidney development.

2018-05-08 | GSE84305 | GEO

Proteomics of FACS-sorted heterogeneous Corynebacterium glutamicum populations

Project description:The metabolic status of individual cells in microbial cultures can differ being relevant for biotechnology, environmental and medical microbiology. However, it is hardly understood in molecular detail due to limitations of current analytical tools. Here, we demonstrate that FACS in combination with proteomics can be used to sort and analyze cell populations based on their metabolic state. A previously established GFP reporter system was used to detect and sort single Corynebacterium glutamicum cells based on the concentration of branched chain amino acids (BCAA) using FACS. A proteomics workflow optimized for small cell numbers was used to quantitatively compare proteomes of a ?aceE mutant, lacking functional pyruvate dehydrogenase (PD), and the wild type. About 800 proteins could be quantified from 1,000,000 cells. In the ?aceE mutant BCAA production was coordinated with upregulation of the glyoxylate cycle and TCA cycle to counter the lack of acetyl CoA resulting from the deletion of aceE.

2017-11-02 | PXD005812 | Pride

Gene expression profile of uniparous mouse mammary cell luminal progenitor subpopulation FACS-sorted based on expression of CD24CD29CD61

Project description:We modeled two types of breastfeeding behaviors in wild type FVB/N mice: (1) normal or gradual involution of breast tissue following prolonged breastfeeding and (2) forced or abrupt involution following short-term breastfeeding. To accomplish this, pups were gradually weaned between 28 and 31 days (gradual involution) or abruptly at 7 days postpartum (abrupt involution). FACS was used to relatively quantify mammary epithelial subpopulations. Gene set enrichment analysis was used to analyze gene expression data from mouse mammary luminal progenitor cells. Briefly, single cell suspension of the mammary glands was enriched for lineage-negative (Lin−) epithelial cells excluding the Lin+ cells, specifically hematopoietic (biotinylated CD45 and TER119), endothelial (biotinylated CD31), and immune (biotinylated BP-1) cells. The negatively selected cell population was enriched for luminal epithelium (LE) and mammary stem cell (MaSC)-enriched/basal epithelium (MaSC-enriched). For FACS analysis, Lin− populations were labeled with CD24-PE, CD29-FITC, and CD61-APC while their respective isotypes were used as negative controls. Cell suspensions were incubated with the appropriate antibodies for 30 min on ice. Cells were resuspended in FACS buffer (1 mM EDTA, 1% HI-FBS, and 25 mM HEPES, pH 7.0 in 1× phosphate-buffered saline) and sorted on a FACS BD LSR II Flow Cytometer.

2019-08-10 | GSE135636 | GEO

RNA-Seq of FACS-sorted cardiomyocytes

Project description:RNA-Seq of FACS-sorted cardiomyocytes

| PRJNA474426 | ENA

RNA-seq on dual-FACS sorted Drosophila Intestinal stem cell and Enteroblast, and effect of SPEN loss of function on gene expression

Project description:Precise regulation of stem cell self-renewal and differentiation properties is essential for tissue homeostasis. Using the adult Drosophila intestine to study molecular mechanisms controlling stem cell properties, we identify the gene split-ends (spen) in a genetic screen as a novel regulator of intestinal stem cell fate. Spen family genes encode conserved RNA recognition motif-containing proteins that are reported to have roles in RNA splicing and transcriptional regulation. We demonstrate that spen loss of function in intestinal stem cells results in an abnormal increase in the number of stem cell-like cells and that Spen acts to control early commitment events of the stem cells. Using two-color cell sorting of stem cells and their daughters, we characterize spen-dependent changes in RNA abundance and exon usage, and find potential key regulators downstream of spen. Our work identifies spen as an important regulator of adult stem cells in the Drosophila intestine, provides new insight to Spen-family protein functions, and may also shed light on Spen’s mode of action in other developmental contexts.

2017-07-20 | GSE84367 | GEO

Human small intestine

Project description:Human small intestine Metagenome

| PRJNA1010206 | ENA