Project description:Food allergy is an immune-mediated condition caused by hypersensitivity to food allergens, with fish allergens, particularly beta-parvalbumins (beta-PRVBs), being the most significant. While the only effective treatment is avoidance of the food allergen, the intracellular mechanisms of immune activation during allergen exposure are not well understood.
This study aimed to investigate critical insights into immune cell activation in a newly proposed beta-PRVB-induced fish allergy model, with a particular focus on to explore the proteomic phenotype of splenocytes and T cell activation in response to the allergen.
To establish a robust allergic response, mice were sensitized to beta-PRVB using aluminium hydroxide, a Th2-skewing adjuvant, (intraperitoneal injection) and subsequently challenged orally with beta-PRVB . This carefully designed protocol was designed to induce a Th2-type immune response, which was confirmed by detecting allergen-specific antibodies and cytokines. For proteomic analysis of the immune response, high-resolution quantitative proteomics data were generated using an advanced DIA-based ZenoSWATH-MS technology.
Project description:PCR array analysis was performed to investigate the expression of genes in the ear tissues of Ni allergy-induced miceand control mice. Ni model mouse n=3, control mouse n=3
Project description:PCR array analysis was performed to investigate the expression of genes in the ear tissues of Ni allergy-induced miceand control mice.
Project description:Comparison of gene expression profiles between pediatric patients with and without symptoms of cross-allergy (birch-apple syndrome).
Project description:Cutaneous exposure to food antigen through impaired skin barrier has been shown to induce epicutaneous sensitization, and thereby cause IgE-mediated food allergy. We examined whether skin barrier impairment deteriorated food allergy symptoms in epicutaneously sensitized mice. To clarify the association between skin inflammation and food allergy symptoms, we analyzed gene expression at skin lesions using a GeneChip.