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High-throughout sequencing of RAW264.7 cells treated with LPS and Harmine

ABSTRACT: High-throughout sequencing of RAW264.7 cells treated with LPS and Harmine

PROVIDER: PRJNA1111748 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29029516_1.fastq.gz	Fastqsanger.gz
	SRR29029516_2.fastq.gz	Fastqsanger.gz
	SRR29029517_1.fastq.gz	Fastqsanger.gz
	SRR29029517_2.fastq.gz	Fastqsanger.gz
	SRR29029518_1.fastq.gz	Fastqsanger.gz

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High-throughout sequencing of RAW264.7 cells treated with LPS and Harmine

Project description:After stimulation of RAW264.7 cells with LPS and Harmine, high-throughput sequencing technology was used to explore the changes in the inflammation level of RAW cells and the enrichment of signaling pathways after LPS and Harmine treatment, and to find an interesting target pathway.

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Project description:In this study, mouse macrophage Raw264.7 was pretreated with Lespedeza bicolor root components and then stimulated with LPS and then high-throughput sequencing technology was used to explore the expression of inflammation-related genes of Raw264.7 in mouse macrophages treated with Lespedeza bicolor root components, and to find interesting targets,NF-kappaB and p38 signaling pathway.

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Project description:We have identified a number of miRNAs that are differentially expressed in LPS and IFNγ treated RAW264.7 cells, as compared to untreated cells. Microarray and quantitative real-time PCR (qRT-PCR) results showed that miR-103 decreased while the STAT1 expression increased substantially in RAW264.7 derived M1 macrophage, and there was a significant negative correlation between miR-103 and STAT1.Overexpression of miR-103 suppressed M1 polarization by inhibiting STAT1/IRF1 signaling pathway and vice versa in vitro. STAT1 is a direct target of of miR-103.

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Project description:RAW264.7 cells were transfected with Synbindin siRNA for 48hours, then 100ng/ml LPS were treated cells for 4 hours. Before and after LPS stimulation, cells were collected and RNA were extracted for RNA-seq.

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Project description:Unanticipated regulatory protein modifications can be discovered from the unbiased comprehensive analysis of biological systems using SAMPEI. To explore this idea, we sought to map protein modifications induced during mammalian cell differentiation, modeled by the response of mouse RAW264.7 macrophage cells to lipopolysaccharide (LPS), a potent inducer of macrophage activation and differentiation that involves extensive protein and metabolic signaling.

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