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Phase Separation of MRG15 delays cellular senescence [CUT&Tag]

ABSTRACT: Phase Separation of MRG15 delays cellular senescence [CUT&Tag]

PROVIDER: PRJNA1146141 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30181182_1.fastq.gz	Fastqsanger.gz
	SRR30181182_2.fastq.gz	Fastqsanger.gz

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Phase Separation of MRG15 delays cellular senescence

Project description:Phase Separation of MRG15 delays cellular senescence

| PRJNA1146139 | ENA

Phase Separation of MRG15 delays cellular senescence [CUT&Tag]

Project description:Phase separation, a biophysical process that segregates subcellular environments into condensates, is recognized for its role in regulating transcription. However, the extent of its influence on cellular senescence processes remains to be fully elucidated. Here, we have established that the depletion of MRG15, a conserved chromodomainprotein, leads to cellular senescence in human mesenchymal stem cells (hMSCs). MRG15 is capable of forming phase-separated liquid condensates via its intrinsically disordered region (IDR). Through the utilization of IDR deletion and replacement assays, we have shed light on the pivotal role that MRG15 condensation plays in the senescence of hMSCs. Through epigenomic and transcriptomic analysis, the depletion of MRG15 impacts pathways that are integral to the cell cycle and the senescence process, as evidenced by the diminished binding and the modified expression of key genes, including CDKN1A, LMNB1, CCNB1, NPM1, MYC, and HMGB2. Within a bleomycin-induced model of idiopathic pulmonary fibrosis in mice, hMSCs of the wild-type variety were able to ameliorate inflammation and fibrosis, a stark contrast to the outcomes observed in MSCs where MRG15 was knocked down or its IDR was deleted. Our findings establishes a link between phase separation and senescence regulation and could present a promising new therapeutic target for the alleviation of age-related diseases and the potential extension of lifespan.

2025-04-02 | GSE274376 | GEO

Phase Separation of MRG15 delays cellular senescence [RNA-Seq]

Project description:Phase Separation of MRG15 delays cellular senescence [RNA-Seq]

| PRJNA1145897 | ENA

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Phase Separation of MRG15 delays cellular senescence

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-04-02 | GSE274377 | GEO

MRG15 interactome upon UV irradiation

Project description:The aim of this analysis is to identify the difference between interaction partners of MRG15 wild type and Chromodomain and MRG domain mutants upon UV irradiation. Therefore, MRG15 wild type and mutants (N-terminal FLAG tag) were overexpressed in U2OS cells. After UV irradiation, immunoprecipitation of the constructs was performed using FLAG M2 affinity gel. The constructs were eluted from the beads with 3xFLAG peptide.

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MRG15

Project description:Drosophila melanogaster MRG15, MORF-related gene 15, is differentially expressed in 12 experiment(s);

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TEAD4 delays cellular senescence by remodeling chromatin accessibility at H3K27ac-occupied enhancer regions [CUT&Tag]

Project description:TEAD4 delays cellular senescence by remodeling chromatin accessibility at H3K27ac-occupied enhancer regions [CUT&Tag]

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Phase separation of YAP-MAML2 differentially regulates the transcriptome

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