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Effect of Cardiac LIM Protein (CSRP3) on human proximal tubule epithelial cells

ABSTRACT: Effect of Cardiac LIM Protein (CSRP3) on human proximal tubule epithelial cells

PROVIDER: PRJNA1156043 | ENA |

REPOSITORIES: ENA

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Effect of Cardiac LIM Protein (CSRP3) on human proximal tubule epithelial cells

Project description:Cardiac LIM protein (CSRP3) is released from the heart after cardiac injury and transits the plasma to the renal filtrate. CSRP3 has a well described transcriptionally-active role in myocardiocyte development and response to stress. Although it appears in endocytic vesicles within tubular epithelial cells in vivo it is unknown whether it alters tubular epithelial cell transcription. Therefore to test the hypothesis that CSRP3 alters PTEC transcription, human primary PTEC were exposed to physiologic concentration of CSRP3 (10nM) for 6h prior to bulk RNA sequencing.

2025-08-20 | GSE276233 | GEO

Pax8 regulates proximal-tubule-specific identity via distal enhancer elements

Project description:Pax8 can reversibly modulate proximal-tubule-specific gene expression and epithelial morphology in cultured proximal tubule cells. Pax8 loss dynamically alters activity of distal enhancer elements but not promoters. These observations strongly suggest that Pax8 regulates proximal tubule phenotype via distal enhancer elements

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RAMPAGE from epithelial cell of proximal tubule (ENCSR861MQP)

Project description:The libraries contained in this experiment come from epithelial cells of proximal tubule (HRPTE piC). They are stranded PE101 Illumina Hi-Seq RAMPAGE libraries from rRNA-depleted Total RNA > 200 nucleotides in size. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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total RNA-seq from epithelial cell of proximal tubule (ENCSR118TVR)

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Profiling of Transcripts in Rat Proximal Tubule

Project description:Freshly isolated rat kidney proximal tubules were subjected for transcript profiling. Three microarray experiments were done to obtain the kidney proxmial tubule transcriptome.

2009-01-26 | E-GEOD-13668 | biostudies-arrayexpress

Effect of GW4064 on primary cultured mouse kidney proximal tubule cells

Project description:Global gene expression in the primary cultured mouse kidney proximal tubule cells treated either DMSO or 1uM GW4064 (a FXR agonist) was compared. Results provide insight into mechanisms underlying effects of FXR activation on gene expression in mouse kidney proximal tubule cells. Male C57/BJ mice aged 6 weeks were sacrificed under anesthesia and kidney proximal tubule cells were cultured until confluent. Cells were treated with either GW4064 (1uM) or equal amount of DMSO and incubated for 24 hours. 4 total RNA samples per group were analyzed and gene expression was compared between the groups.

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Copper-responsive proteome changes in human kidney proximal tubule epithelial cells

Project description:Copper is essential for cellular function but potentially toxic in excess. While copper's influence on cytoskeletal dynamics remains unclear, loss of mechanical integrity is a hallmark of kidney disease. Here we investigated how copper availability modulates mechanical properties and cytoskeletal architecture of human proximal tubule epithelial cells (HK-2). Cells were treated for 24h with 50 µM CuCl₂ (copper supplementation) or 10 µM tetrathiomolybdate (TTM, copper chelation). Quantitative proteomics revealed coordinated changes in actin-regulatory proteins including Rab35, Septin2, MsrB2, and CK2 subunits, establishing redox-dependent pathways linking copper homeostasis to cytoskeletal dynamics. A total of 4,933 proteins were quantified with 89-90% data completeness, including detection of 13,919 PTMs per run (carbamidomethylation, oxidation, phosphorylation sites)

2026-07-21 | PXD072220 | Pride

Transcriptomes of Major Proximal-Tubule Cell-Culture Models

Project description:Purpose:Cultured cell lines are widely used for research in the physiology, pathophysiology, toxicology and pharmacology of the renal proximal tubule. The lines that are most appropriate for a given use depend on the genes expressed.We have used modern RNA-sequencing techniques to identify the gene expression profile of 14 different cell lines plus primary cultures of mouse proximal tubule and compare them to transcriptomes of native kidney proximal tubules. Methods: 14 different proximal tubule cell lines were grown on permeable supports under conditions specific for the respective lines. RNA-Seq followed standard procedures. Results and conclusion: Transcripts expressed in cell lines showed variable match to transcripts selectively expressed in native proximal tubule. Opossum kidney (OK) cells displayed the highest percentage match (45%) with pig kidney cells (LLC-PK1) close behind (39%). Much lower percentage matches were seen for various human lines including HK-2 cells (26%) and lines from rodent kidneys (18-23%).An online resource (https://esbl.nhlbi.nih.gov/JBrowse/KCT/) has been created for interrogation of the data.No cell line closely matched the transcriptome of native proximal tubule cells. However, some of the lines tested are suitable for the study of particular metabolic and transport processes seen in the proximal tubule.

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Effects of PAX8 depletion on gene expression in proximal tubule epithelial cells.

Project description:Effects of PAX8 depletion on gene expression in proximal tubule epithelial cells.

| PRJNA1405761 | ENA