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Single-cell RNA sequencing analysis of E13.5 mouse skin data

ABSTRACT: Single-cell RNA sequencing analysis of E13.5 mouse skin data

PROVIDER: PRJNA1180616 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31187394.fastq.gz	Fastqsanger.gz
	SRR31187395.fastq.gz	Fastqsanger.gz

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Single-cell RNA sequencing analysis of E13.5 mouse skin data

Project description:Mouse embryo skin samples were collected around embryonic day 13.5 (E13.5). Cells from the wildtype and the SmoM2YFP mutant were pooled for analyses.

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Single-cell RNA-seq of the developing C57/BL6 mouse back skin at E13.5, E16.5 and P0

Project description:We performed single-cell RNA seq on C57/BL6 mouse back skin at E13.5, E16.5, and P0 to study embryonic hair follicle development. We analyzed 15,086 single cell transcriptome profiles from E13.5, E16.5 and newborn mice (postnatal day 0, P0) dorsal skin cells across hair follicle induction, organogenesis, cytodifferentiation stage. Based on t-distributed Stochastic Neighbor Embedding (tSNE) clustering, we identified 14 cell clusters from skin cells and delineated their cell identity gene expression profile. By using Monocle pseudotime ordering analysis, we constructed epithelium/dermal cell lineage differentiation trajectory and revealed sequential activation of key regulons involved during embryonic hair follicle morphogenesis. Our findings here provide molecular landscape during hair follicle epithelium/dermal cell lineage fate decisions.

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Single cell sequencing of dissected mouse mandibular prominence at embryonic day e11.5 and e13.5

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Single-cell RNA sequecing of E13.5 mouse cerebella

Project description:Single-cell RNA sequecing of E13.5 mouse cerebella

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Single-cell RNA sequecing of E13.5 mouse cerebella

Project description:In this study we performed single-cell sequencing of E13.5 mouse cerebella, revealing different newly generated neurons and their associated molecular features.

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Single-cell RNA sequencing analysis of E14.5 mouse skin data

Project description:Single-cell RNA sequencing analysis of E14.5 mouse skin data

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Single cell sequencing of the E13.5 mouse anterior palate reveals mesenchymal heterogeneity

Project description:Cleft palate is one of the most prevalent birth defects. Mice are useful for studying palate development because of their morphological and genetic similarities to humans. In mice, palate development occurs between embryonic days (E)11.5 to 15.5. Single cell transcriptional profiles of palate cell populations have been a valuable resource for the craniofacial research community, but we lack a single cell transcriptional profile for anterior palate at E13.5, at the transition from proliferation to shelf elevation. Here, a detailed single cell RNA sequencing analysis reveals heterogeneity in expression profiles of the cell populations of the E13.5 anterior palate. Mesenchymal populations spatially segregate into four domains. One of these mesenchymal populations expresses ligands and receptors distinct from the rest of the mesenchyme, suggesting that these cells have a unique function. RNAVelocity analysis shows two terminal cell states that contribute to either the proximal or distal palatal regions emerge from a single progenitor pool. This single cell resolution expression data and detailed analysis from E13.5 anterior palate provides a powerful resource for mechanistic insight into secondary palate morphogenesis for the craniofacial research community.

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Single-cell RNA-seq of the developing C57/BL6 mouse back skin at E13.5, E16.5 and P0

Project description:Single-cell RNA-seq of the developing C57/BL6 mouse back skin at E13.5, E16.5 and P0

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Single cell sequencing of the E13.5 mouse anterior palate reveals mesenchymal heterogeneity

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