Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA118075 | ENA |
REPOSITORIES: ENA
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Project description:Mesenchymal stem/stromal cells (MSCs) were harvested from subcutaneous adipose tissue of patients with obesity or healthy controls and expanded for 3-4 passages, and 5hmC profiles were examined through hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq). We hypothesized that obesity and cardiovascular risk factors induce functionally-relevant, locus-specific changes in overall exonic coverage of 5hmC in human adipose-derived MSCs.
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Project description:In this study, the proteome and metabolic properties of primary subcutaneous (s) and visceral (v) adipose tissue-derived stromal stem cells (ASC) from old and young rabbits were analysed by a mass spectrometry-based label-free quantification approach and combined with metabolic mitochondrial respiration measurements (Seahorse Mito Cell Stress Test, Agilent).
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Project description:Adipose-derived stromal/stem cells (ASC) capable of multipotential differentiation can be isolated with high yield from human subcutaneous lipoaspirates. This study reports our recent experience isolating and immunophenotypically characterizing ASCs from >60 human subjects
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Project description:Pathological expansion of adipose tissue (AT) in obesity is supported by adipocyte precursors, termed adipose-derived stromal/stem cells (ASCs). Elucidation of mechanisms underlying ASC function may lead to therapeutic interventions to treat fat mass accumulation. Using epigenome-wide association studies, we explored the impact of obesity on the methylation signature of human ASCs.
2019-12-05 | GSE111632 | GEO
Project description:The therapeutic potential of mesenchymal stem/stromal cells (MSCs) relies on their ability to modulate immune responses in disease and wound contexts. In this study, we present an analytical pipeline using established algorithms to better characterize the response of adipose-derived stem/stromal cells (ASCs) to in vitro inflammatory stimuli, which in turn has therapeutic benefit in the context of MSC cell therapies. By integrating single-cell RNA sequencing and bulk proteomics data of ASCs derived from the stromal vascular fraction of adipose tissue, we present a detailed characterization of the ASC response to inflammation and additionally introduce a method of relating phenotypes observed in exogenously stimulated culture conditions to subpopulations of in vivo cells. Our pipeline involves multiple benchmarks to establish concordance between single-cell and bulk assays to more comprehensively characterize the ASC response to inflammation. This study is the introduction of a practical and efficient approach to identify functionally homogeneous and therapeutically relevant cell populations from heterogeneous mixtures using computational strategies and provides deeper insight into the ASC response to inflammation.
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Project description:Transcriptome Sequencing of Adipose-Derived Mesechymal Stromal Cells
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Project description:Effect of monocyte- and osteoclast-derived extracellular vesicles on adipose tissue-derived mesenchymal stem/stromal cells
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Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Cystic kidney disease (CKD) is a heterogeneous group of genetic disorders and one of the most common causes of end-stage renal disease. Here, we investigate the potential effects of long-term human stem cells treatment on kidney function and the gene expression profile of PKD/Mhm (Cy/+) rats. Human adipose derived stromal cells (ASC) and human skin-derived ABCB5+ stromal cells (2x106) were monthly, over a period of 6 months, infused intravenously or injected intraperitoneally. Additionally, ASC and ABCB5+ derived conditioned media were administrated intraperitoneally. Gene expression profile results showed a significant reprogramming of metabolism related pathways along with the down-regulation of cAMP, NF-B and apoptosis pathways. During the experimental period, the principal renal parameters as well as renal function using an innovative non-invasive transcutaneous device were measured. All together, these analyses show a moderate amelioration of the renal function in ABCB5+ and ASC treated groups. Additionally, ABCB5+ and ASC derived conditioned media treatments lead to milder, but still promising improvements. Cell-based therapy may constitute a novel therapeutic approach in CKD.
2022-05-18 | GSE161092 | GEO
Project description:Cystic kidney disease (CKD) is a heterogeneous group of genetic disorders and one of the most common causes of end-stage renal disease. Here, we investigate the potential effects of long-term human stem cells treatment on kidney function and the gene expression profile of PKD/Mhm (Cy/+) rats. Human adipose derived stromal cells (ASC) and human skin-derived ABCB5+ stromal cells (2x106) were monthly, over a period of 6 months, infused intravenously or injected intraperitoneally. Additionally, ASC and ABCB5+ derived conditioned media were administrated intraperitoneally. Gene expression profile results showed a significant reprogramming of metabolism related pathways along with the down-regulation of cAMP, NF-B and apoptosis pathways. During the experimental period, the principal renal parameters as well as renal function using an innovative non-invasive transcutaneous device were measured. All together, these analyses show a moderate amelioration of the renal function in ABCB5+ and ASC treated groups. Additionally, ABCB5+ and ASC derived conditioned media treatments lead to milder, but still promising improvements. Cell-based therapy may constitute a novel therapeutic approach in CKD.
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