Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1215922 | ENA |
REPOSITORIES: ENA
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Project description:Tire-wear particles (TWPs) are considered among the largest contributors of microplastics to the environment. They are subject to break-down due to environmental weathering, which allows for potentially toxic chemicals to be leached from and sorbed onto the particles. In this study, leachate generated from “weathered” and “un-weathered” TWPs were used for sublethal toxicity tests with Americamysis bahia.
2025-01-01 | GSE223584 | GEO
Project description:Tire wear particles (TWP) are small micro- or nano-particles resulting from the friction of tire tread against roads. These microplastics have been found in waterways, usually through rain and wind, posing a potential risk to exposed aquatic life. The goal of this project was to assess the toxicity of chemical leachates from TWP and characterize how they may impact the health and development of aquatic life, like Danio rerio, zebrafish. Furthermore, we also determined if solar-simulated or dark conditions intensified the effects of TWP, considering processes like photodegradation and photochemical oxidation occur in sunlight. Zebrafish were exposed from 0-4 days post fertilization (dpf) to a filtered TWP solution at varying concentrations, and each concentration was leached in either solar-simulated or dark conditions. Mortality and hatching rates were quantified throughout the exposures, and physical anomalies were recorded at 4 dpf using microscopy. Ethyoxyresorufin-O-deethylase (EROD) activity, an indicator of Phase I biotransformation, was performed using fluorescent microscopt. RNA sequencing was performed for 4 dpf larvae. TWP exposures, regardless of leaching light status, increased mortality, yolk sac edema, pericardial edema, spinal curvature, and craniofacial malformations, while decreasing hatching and swim bladder inflation. Regardless of lighting during leaching, EROD activity increased in the liver and brain and decreased in the gut CYP1A. RNA sequencing revealed some conserved processes in response to TWP, regardless of light status during leaching, including those involved in neurobiological function. However, divergent responses were also observed for processes such as amino acid metabolism, steroid biosynthesis, and fatty acid metabolism. Overall, these results suggest a disruption of embryonic development in zebrafish when exposed to TWP, and that sunlight during chemical leaching can alter the molecular responses to TWP.
2025-08-25 | GSE233518 | GEO
Project description:Tire-wear particle leachate toxicity to Americamysis bahia: analysis of sublethal and molecular effects
| PRJNA926920 | ENA
Project description:Recently, omics techniques have been widely applied to the discovery of potential bio-markers and explore triggering mechanism. To get a more comprehensive diagnosis of HBCD impacts on marine medaka (Oryzias melastigma), the larvae (within 24 hours post-hatch) were exposed to gradient doses of HBCD. After exposure for 7 days, the profiles of genes expression were examined using a custom-commercial 26, 430-oligonucleotide arrays (4M-CM-^W44K) of Japanese medaka which is shared much genomic information with marine medaka.At the end of the treatment period, 30 larvae/sample were pooled for RNA extraction and labeled by One-Color. A total of twelve independent arrays: three control (DMSO), three low-concentration HBCD (0.2 nM) exposures, three medium-concentration HBCD (2 nM) exposures, and three high-concentration HBCD (20 nM) exposures. The larvae of marine medaka (within 24 hours post-hatch) were exposed to to 0 (control), 0.2nM, 2nM and 20nM of HBCD (dimethyl sulfoxide with a final concentration of 1:30000 v/v water) for 7 days. Each HBCD treatment had three replicates with 100 larvae for each Petri dish. At the end of the treatment period, 30 larvae/sample were pooled for RNA extraction. A total of twelve independent arrays: three control (DMSO), three low-concentration HBCD (0.2 nM) exposures, three medium-concentration HBCD (2 nM) exposures, and three high-concentration HBCD (20 nM) exposures.
2013-07-31 | E-GEOD-49362 | biostudies-arrayexpress
Project description:We sequenced mRNA extracted from brains of (1) D. melanogaster larvae exposed to food containing 5% ethanol (v/v) for 6 conscutive days, and (2) an age-matched untreated control larvae, that grew in regular food. Differential gene expression between the two groups was calculated and reported. Each group consisted of 3 biological replicates of 30 brains each.
2021-10-13 | GSE185625 | GEO
Project description:Recently, omics techniques have been widely applied to the discovery of potential bio-markers and explore triggering mechanism. To get a more comprehensive diagnosis of HBCD impacts on marine medaka (Oryzias melastigma), the larvae (within 24 hours post-hatch) were exposed to gradient doses of HBCD. After exposure for 7 days, the profiles of genes expression were examined using a custom-commercial 26, 430-oligonucleotide arrays (4×44K) of Japanese medaka which is shared much genomic information with marine medaka.At the end of the treatment period, 30 larvae/sample were pooled for RNA extraction and labeled by One-Color. A total of twelve independent arrays: three control (DMSO), three low-concentration HBCD (0.2 nM) exposures, three medium-concentration HBCD (2 nM) exposures, and three high-concentration HBCD (20 nM) exposures.
2013-07-31 | GSE49362 | GEO