Project description:Tire-wear particles (TWPs) are considered among the largest contributors of microplastics to the environment. They are subject to break-down due to environmental weathering, which allows for potentially toxic chemicals to be leached from and sorbed onto the particles. In this study, leachate generated from “weathered” and “un-weathered” TWPs were used for sublethal toxicity tests with Americamysis bahia.
2025-01-01 | GSE223584 | GEO
Project description:The effects of tire wear particles on zebrafish eyes
Project description:Rationale Microplastics are a pressing global concern and inhalation of microplastic fibers has been associated with interstitial and bronchial inflammation in flock workers. However, how microplastic fibers affect the lungs is unknown. Objectives Our aim was to assess the effects of 12x31 µm nylon 6,6 (nylon) and 15x52 µm polyethylene terephthalate (polyester) textile microplastic fibers on lung epithelial growth and differentiation. Methods We used human and murine alveolar and airway-type organoids as well as air-liquid interface cultures derived from primary lung epithelial progenitor cells and incubated these with either nylon or polyester fibers or nylon leachate. In addition, mice received one dose of nylon fibers or nylon leachate and 7 days later organoid-forming capacity of isolated epithelial cells was investigated. Results We observed that nylon microfibers, more than polyester, inhibited developing airway organoids and not established ones. This effect was mediated by components leaching from nylon. Epithelial cells isolated from mice exposed to nylon fibers or leachate, also formed fewer airway organoids, suggesting long-lasting effects of nylon components on epithelial cells. Part of these effects were recapitulated in human air-liquid interface cultures. Transcriptome analysis revealed upregulation of Hoxa5 post-exposure to nylon fibers. Inhibiting Hoxa5 protein during nylon exposure restored airway organoid formation, confirming Hoxa5's pivotal role in the effects of nylon. Conclusions These results suggest that components leaching from nylon 6,6 may especially harm developing airways and/or airways undergoing repair and we strongly encourage to characterize both hazard of and exposure to microplastic fibers in more detail.
Project description:This dataset contains results for a semi-untargeted metabolomics study on fungal metabolite identification of the tire wear compounds acetanilide and hexamethoxymethylmelamine. High-resolution Orbitrap mass spectrometry was used along with Compound Discoverer (version 3.1) for downstream analysis. Further information is available in the readme file and related publication.
Project description:ngs2014_07_hevea-hevea_tpd-seq-RNAseq analysis of latex samples from healthy and Tapping Panel Dryness-affected trees.-Identification of Tapping Panel Dryness (TPD)-affected trees in a polyclonal trials grown under standard condition. Trees were tapped since November 2010 every 2 days. Latex yield and TPD occurrence were monitored as well as latex RNA samples were collected twice a year for further analysis. At the end of the experiment, gene expression in latex of healthy and TPD trees were compared.
Project description:Effects of tire wear particles on freshwater bacterial-fungal community dynamics and subsequent elemental cycles using microcosms Raw sequence reads
