Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1227421 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR32465622_1.fastq.gz | Fastqsanger.gz | |||
| SRR32465622_2.fastq.gz | Fastqsanger.gz | |||
| SRR32465623_1.fastq.gz | Fastqsanger.gz | |||
| SRR32465623_2.fastq.gz | Fastqsanger.gz | |||
| SRR32465624_1.fastq.gz | Fastqsanger.gz |
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Project description:Background: The anterior pituitary plays a crucial role in endocrine regulation and homeostasis by orchestrating hormonal signaling, cellular growth, and functional adaptations. Adipokines regulate hypothalamic-pituitary-ovarian axis influencing hormone secretion and cellular processes. Omentin-1, an adipokine primarily expressed in visceral adipose tissue, is involved in metabolic and reproductive functions. However, its role in pituitary remains unknown. We hypothesize that omentin-1 modulates protein abundance in pituitary cells. This study aimed to investigate role of omentin-1 in regulating key pathways in anterior pituitary cells of pigs with different metabolic statuses: normal-weight Large White pigs and genetically obese Meishan pigs. Results: High-throughput proteomic analysis of porcine anterior pituitary cells exposed to omentin-1 revealed 230 differentially abundant proteins (30 downregulated and 3 upregulated in Large White pigs, as well as 107 downregulated and 90 upregulated in Meishan pigs), associated with 116 gene ontology terms. The most significantly altered proteins were associated with cell cycle and DNA replication, gene expression regulation and posttranscriptional/posttranslational modifications. Apoptotic and proliferative mechanisms varied between breeds, suggesting different metabolic adaptation strategies. In Large White pigs, CDK5RAP2 and SIX1 played a key role in transcriptional and proliferative regulation, whereas in Meishan pigs, stronger proliferative control was linked to AKT1S1 and RHOA. Moreover, Meishan pigs demonstrated a more dynamic regulation of apoptotic pathways (HTRA2, PARP2, DFFA), which may promote greater metabolic plasticity and better adaptation to physiological fluctuations. Additional in vitro experiments in Large White pigs showed that omentin-1 influenced key proliferative and apoptotic markers, downregulating cyclins D1, E1, A2, and B1, as well as caspase-3, while upregulating the anti-apoptotic protein BCL2, leading to an increased BCL2/BAX ratio. Further, omentin-1 modulated phosphorylation of selected kinases, including ERK1/2, AKT, AMPKα, and STAT3, in a time-dependent manner. It is also increased insulin receptor expression and promoted packaging and transport of secretory proteins. Conclusions: Proteomic and functional analyses revealed that omentin-1 regardless of breed exerts anti-proliferative and anti-apoptotic effects on porcine anterior pituitary cells, modulating key signaling pathways. These findings highlight that omentin-1 is a potential metabolic regulator influencing anterior pituitary function.
2025-06-17 | PXD061269 | Pride
Project description:Granulosa cells (Gc) in the ovary play a vital role in nurturing oocyte development, producing estrogens, and maintaining the hormonal balance necessary for follicle maturation and successful ovulation. Omentin-1 (ITLN1) is an adipokine involved in energy metabolism and insulin resistance; its expression has been demonstrated in the ovary and varies depending on the degree of pig fatness. However, its effect on the global proteome of Gc has not been previously investigated. It was hypothesized that ITLN1 affects the abundance of proteins involved in key processes occurring in Gc during the mid-luteal phase of the estrous cycle in normal weight Large White and fat Meishan pigs, with potential variation based on the fat content of the pigs. Liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) analysis of porcine Gc identified 208 statistically significant differentially abundant proteins (DAPs) in the Large White pigs, with 99 proteins upregulated, and 109 downregulated. In Meishan pigs, 42 statistically significant DAPs were identified, including 25 upregulated and 17 downregulated proteins. Western blotting and ELISA analyses of six selected DAPs (cadherin 5, farnesyl diphosphate synthase, fibroblast growth factor receptor 1, matrix metallopeptidase 2, phosphatidylinositol-5-phosphate 4-kinase type 2 gamma, and yes-associated protein 1) confirmed the accuracy and precision of the LC‒MS/MS methodology. The identified DAPs were associated with several key biological processes, including the estrogen signaling pathway, cell cycle and DNA replication, protein synthesis, transport and maturation, cytoskeleton dynamics, cell signaling, and hormonal regulation. The results indicate that ITLN1 has a modulatory influence on the porcine Gc proteome, which is dependent on fat content. This highlights the important role of ITLN1 in regulating ovarian functions.
2025-07-23 | PXD061976 | Pride
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs.
2009-06-20 | GSE11853 | GEO
Project description:From gestation day 75 to gestation day 90, an important stage for the placental and fetal development, the fetuses grow rapidly and need adequate nutrition. The Meishan pigs and the Large White pigs employ different ways in supplying the enough nutrients and oxygen to the fetus. The Meishan pigs increased the vascular density and the Large White pigs have the second increase in the surface of placenta. To understand the molecular basis related to late gestation placenta development in Chinese indigenous and Western breeds with different placental efficiency, samples were collected and used to hybridized. The results offered new data on understanding the molecular basis of placenta efficiency, and indicated that Erhualian pigs had the more efficient than the Large White pigs. Experiment Overall Design: Placenta efficiency (the body weight of a piglet divided by the mass of its placenta) of Erhualian pigs is markedly higher than Large White pigs. so placenta samples (female) from 6 Erhuanlian pregnant gilts at gestation day 75 (E75) and day 90 (E90) and 6 Large White pregnant gilts at gestation day 75 (L75) and day 90 (L90) were collected. RNAs from two female piglet placentas from each gilt were combined to 12 pools and hybridized to the porcine Affymetrix GeneChip.
2009-07-01 | E-GEOD-11853 | biostudies-arrayexpress
Project description:We collected the ovary samples at 49 days post coitus in Large White and Meishan adult female pigs, identified the differentially expressed protein coding genes and long non-coding RNAs between these two breeds. three individuals of each breed were harvested as biological replicates.
2017-06-12 | GSE73593 | GEO
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
2008-01-07 | GSE8377 | GEO
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples
2010-06-29 | E-GEOD-8377 | biostudies-arrayexpress