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Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

ABSTRACT: Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

PROVIDER: PRJNA1241251 | ENA |

REPOSITORIES: ENA

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Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

Project description:We identified a novel M. bovis nucleomodulin, MbovP202, which functions as a 5-methylcytosine-specific DNA methyltransferase that localizes to the nucleus of the bovine macrophage cell line (BoMac). Whole genome bisulfite sequencing (WGBS) demonstrated that MbovP202 increases methylation at mCATG sites and mCG sites. Integrated analysis of WGBS and RNA-Seq data revealed that genes with promoter hypermethylation and concomitant down-regulated expression were predominantly enriched in metabolic and inflammatory pathways.

2025-04-30 | GSE292785 | GEO

Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

Project description:We identified a novel M. bovis nucleomodulin, MbovP202, which functions as a 5-methylcytosine-specific DNA methyltransferase that localizes to the nucleus of the bovine macrophage cell line (BoMac). To investigate its regulatory role, we performed RNA sequencing to compare the mRNA expression profiles between BoMac and BoMacMbovP202 cells. A total of 2,790 differentially expressed genes (DEGs) were identified between the two groups. Integrated analysis of Whole Genome Bisulfite Sequencing (WGBS) and RNA-Seq data revealed that genes with promoter hypermethylation and concomitant down-regulated expression were predominantly enriched in metabolic and inflammatory pathways.

2025-04-20 | GSE292360 | GEO

Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

Project description:Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

| PRJNA1238232 | ENA

microRNA identification in the Mycoplasma bovis infected mammary gland tissue in Holstein Friesian cattle

Project description:The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells and, endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, mammary gland tissue samples were collected from Holstein cows and screened for Mycoplasma bovis. Then, total RNA was isolated from mycoplasma bovis infected tissues and RNA sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. Our results revaled that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis case caused by Mycoplasma bovis.

2021-05-25 | GSE152865 | GEO

Genome-wide maps of MbovP475-binding sits in BoMac cells.

Project description:We used Next-generation sequencing (NGS) to reveal MbovP475-binding sites in BoMac cells. We extracted the genome of BoMac infected with mycoplasma bovis by sonication, then the sheared chromatin in the supernatant was incubated with purified rabbit polyclonal IgG against MbovP475 and protein A/G magnetic beads. The DNA was eluted from the beads and subject to making sequencing library. We totally got 2648 potential MbovP475-binding sites by ChIP-seq. Among them, CRYAB and MCF2L2 gene were further verifed to be down-reguated by MbovP475 through binding to the promoter region of two genes.

2021-07-14 | GSE179651 | GEO

Comprehensive Immunoproteome of Mycoplasma bovis

Project description:This study was aimed to elucidate a global antigenic profile of Mycoplasma bovis (M. bovis) with immunoproteomics, immunoinformatics, and gene expression approaches. The extracts of whole-cell proteins and TX-114 membrane fraction of a Chinese strain M. bovis HB0801 were separated with two dimensional gel electrophoresis (2-DE) and proteins reacting with antisera to M. bovis from experimentally infected calves were detected by MALDI-TOF MS.

2016-06-13 | PXD003479 | Pride

Transcription profiling of bovine peripheral blood mononuclear cells in intramammary infusion of Mycoplasma bovis

Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine peripheral blood mononuclear cells play an important role for mycoplasma mastitis, however, the effects of M. bovis for immune response of peripheral blood mononuclear cells have not been fully clarified.We examined the transcription profiling of bovine peripheral blood mononuclear cells in intramammary infusion of M. bovis at day 7.

2019-12-19 | E-MTAB-7905 | biostudies-arrayexpress

Transcription profiling of bovine neutrophils stimulated with Mycoplasma bovis against untreated controls

Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine neutrophils play an important role for the eradication of pathogens which cause mycoplasmal infection, however the effects of M. bovis for immune response of neutrophils have not been fully clarified. We examined the transcription profiling of bovine neutrophils on the stimulation with M. bovis for 3h (3 stimuli, 3 control).

2020-11-30 | E-MTAB-9022 | biostudies-arrayexpress

Differential expressed proteins between Mycoplasma bovis different strains

Project description:Comparing differential expressed proteins between Mycoplasma bovis different strains by using label-free method

2020-06-15 | PXD017374 | Pride

Transcription profiling of bovine mammary epithelial cells stimulated with Mycoplasma bovis against untreated controls

Project description:Mycoplasma species are highly contagious pathogens, and intramammary Mycoplasma infection is a serious issue for the dairy industry. The bovine mammary epithelial cells (bMEC) play an important role for the eradication of pathogens which cause intramammary infection, however the effects of M. bovis for immune response of bMEC have not been fully clarified. We examined the transcription profiling of bMEC on the stimulation with M. bovis for 6h (3 stimuli, 3 control).

2017-11-30 | E-MTAB-5306 | biostudies-arrayexpress

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