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Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

ABSTRACT: We identified a novel M. bovis nucleomodulin, MbovP202, which functions as a 5-methylcytosine-specific DNA methyltransferase that localizes to the nucleus of the bovine macrophage cell line (BoMac). To investigate its regulatory role, we performed RNA sequencing to compare the mRNA expression profiles between BoMac and BoMacMbovP202 cells. A total of 2,790 differentially expressed genes (DEGs) were identified between the two groups. Integrated analysis of Whole Genome Bisulfite Sequencing (WGBS) and RNA-Seq data revealed that genes with promoter hypermethylation and concomitant down-regulated expression were predominantly enriched in metabolic and inflammatory pathways.

ORGANISM(S): Bos taurus

PROVIDER: GSE292360 | GEO | 2025/04/20

REPOSITORIES: GEO

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Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

Project description:We identified a novel M. bovis nucleomodulin, MbovP202, which functions as a 5-methylcytosine-specific DNA methyltransferase that localizes to the nucleus of the bovine macrophage cell line (BoMac). Whole genome bisulfite sequencing (WGBS) demonstrated that MbovP202 increases methylation at mCATG sites and mCG sites. Integrated analysis of WGBS and RNA-Seq data revealed that genes with promoter hypermethylation and concomitant down-regulated expression were predominantly enriched in metabolic and inflammatory pathways.

2025-04-30 | GSE292785 | GEO

Effect of MbovP475 on gene expression in BoMac cells

Project description:MbovP475 of M. bovis is a nucleomodulin and has the ability to enter into cells nucleus during infection. Recently, we verifed that MbovP475 can regulate the hostone PTMs. To detect the effect of MbovP475 of M. bovis on gene expression in BoMac cells, we constructed BoMac cells stablely expressing MbovP475. Our data provide globaly change of mRNA profile caused by MbovP475.

2026-06-03 | GSE278667 | GEO

Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

Project description:Mycoplasma bovis nucleomodulin MbovP202 modulates gene expression in BoMac cells

| PRJNA1238232 | ENA

Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

Project description:Mycoplasma bovis nucleomodulin MbovP202 modulates DNA methylation in BoMac cells

| PRJNA1241251 | ENA

Effect of M . bovis NX2-EVs on gene expression in BoMac cells

Project description:Our research shows that EVs derived from M. bovis-infected BMEC cells (EVs-M. bovis NX2) could regulate BoMac cell injury by inducing inflammation. To detect the effect of EVs-M. bovis NX2 on gene expression in BoMac cells, we incubated Ctrl-EVs and EVs-M. bovis NX2 with BoMac cells for 12h, 24h. Our data provide globaly change of mRNA profile caused by EVs-M. bovis NX2.

2025-11-14 | GSE281774 | GEO

Effect of MbovP475 on gene expression in BoMac cells [ChIP-seq]

Project description:MbovP475 of M. bovis is a nucleomodulin and has the ability to enter into cells nucleus during infection. Recently, we verifed that MbovP475 can regulate the hostone PTMs. We found MbovP475 promoted the H3K4me3 and H3K27ac. So we proposed MbovP475 regulated gene expression in BoMac cells by promoting H3K4me3 and H3K27a, we constructed BoMac cells stablely expressing MbovP475. Our data provide globaly genes marked by H3K4me3 and H3K27ac enhanced by MbovP475.

2026-06-03 | GSE278712 | GEO

RNA-seq was used to determine the miRNAs expression profiles of Ctrl-EVs and M. bovis NX2-EVs.

Project description:In this work, we detect the effect of EVs derived from M. bovis-infected BMEC cells (M. bovis NX2-EVs) on regulating the inflammatory response of BoMac. The results indicate M. bovis-infected BMEC cells promote the inflammatory response in BoMac cells by releasing EVs. We furtherly identify the miRNAs in EVs involved in promoting inflammatory response. Understanding their cargo content will provide new insights into the molecular mechanisms of these respective EVs.

2025-11-14 | GSE281987 | GEO

Genome-wide maps of MbovP475-binding sits in BoMac cells.

Project description:We used Next-generation sequencing (NGS) to reveal MbovP475-binding sites in BoMac cells. We extracted the genome of BoMac infected with mycoplasma bovis by sonication, then the sheared chromatin in the supernatant was incubated with purified rabbit polyclonal IgG against MbovP475 and protein A/G magnetic beads. The DNA was eluted from the beads and subject to making sequencing library. We totally got 2648 potential MbovP475-binding sites by ChIP-seq. Among them, CRYAB and MCF2L2 gene were further verifed to be down-reguated by MbovP475 through binding to the promoter region of two genes.

2021-07-14 | GSE179651 | GEO

Comprehensive Immunoproteome of Mycoplasma bovis

Project description:This study was aimed to elucidate a global antigenic profile of Mycoplasma bovis (M. bovis) with immunoproteomics, immunoinformatics, and gene expression approaches. The extracts of whole-cell proteins and TX-114 membrane fraction of a Chinese strain M. bovis HB0801 were separated with two dimensional gel electrophoresis (2-DE) and proteins reacting with antisera to M. bovis from experimentally infected calves were detected by MALDI-TOF MS.

2016-06-13 | PXD003479 | Pride

The bovine alveolar macrophage (bAM) DNA methylome is resilient to infection with Mycobacterium bovis

Project description:Whole genome bisulfite sequencing (WGBS) was used to assess the effect of M. bovis infection on the bAM DNA methylome (5-methylcytosine). The methylomes of bAM infected with M. bovis (n = 8) were compared to those of non-infected control bAM (n = 8) at 24 hours post-infection (hpi). No differences in DNA methylation (CpG or non-CpG) were observed between control and infected bAM. Analysis of DNA methylation at proximal promoter regions uncovered >250 genes harbouring intermediately methylated (IM) promoters (average methylation = 33–66%). Gene ontology (GO) analysis, focusing on genes with low, intermediate or highly methylated promoters, revealed that genes with IM promoters were enriched for immune-related GO categories; this enrichment was not observed for genes in the high or low methylation groups. Targeted analysis of two non-imprinted genes in the IM category, C1QB and IL2RA, confirmed the WGBS observation. This study is the first in cattle to examine genome-wide DNA methylation at single nucleotide resolution in an important bovine cellular host-pathogen interaction model and provides evidence for intermediate promoter methylation in bAM.

2019-02-13 | GSE110412 | GEO

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