Project description:We conducted 16S rRNA sequencing analyse on colonic contents to evaluate whether forced loss led to alterations in gut microbiota composition and function.
Project description:The gut microbiota plays a critical role in host energy metabolism and immune regulation, yet the temporal dynamics of microbial community responses to acute physiological stressors remain poorly characterized. While exercise is recognized as a modulator of gut microbial composition, the immediate post-exercise shifts in microbial community structure across different genetic backgrounds have not been systematically examined. Here, we present a longitudinal 16S rRNA gene sequencing dataset profiling the temporal dynamics of gut microbiota following acute high-intensity exercise in two widely used laboratory mouse strains. Age-matched male BALB/c and C57BL/6J mice were subjected to a single bout of acute exercise. The colonic content samples were collected to capture early temporal responsesat. Total genomic DNA was extracted from colonic contents and the 16S rRNA V4 region was amplified and sequenced on the NovaSeq6000 platform. This dataset enables identification of strain-specific and conserved exercise-responsive microbial taxa, and supports functional predictions related to energy metabolism and intestinal homeostasis.
2026-03-31 | GSE324758 | GEO
Project description:16S rRNA gene sequencing of colonic contents after stress
| PRJNA805133 | ENA
Project description:16S rRNA sequencing of mice feces or colonic contents
Project description:Interventions: Case (colorectal cancer) group:a newly diagnosed colorectal cancer( CRC ) by colonoscopy and pathology;Control group:Clinically healthy volunteers with no symptoms or history of intestinal disease(e.g. colonic adenomatous polyps, CRC or inflammatory bowel disease)
Primary outcome(s): composition of gut microbiota;intestinal microbial phytase activity;16s rRNA metagenomic sequencing;diet surveys;phytic acid intake
Study Design: Case-Control study
| 2667847 | ecrin-mdr-crc
Project description:16S rRNA gene sequencing of mouse colonic contents microbial community
Project description:We compared the microbiota of paired mouse caecal contents and faeces by applying a multi-omic approach, including 16S rDNA sequencing, shotgun metagenomics, and shotgun metaproteomics. The aim of the study was to verify whether faecal samples are a reliable proxy for the mouse colonic luminal microbiota, as well as to identify changes in taxonomy and functional activity between caecal and faecal microbial communities, which have to be carefully considered when using stool as sample for mouse gut microbiota investigations.
