Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Ultra-deep bulk RNA-seq from hESC-derived thyroid follicular cells

ABSTRACT: Ultra-deep bulk RNA-seq from hESC-derived thyroid follicular cells

PROVIDER: PRJNA1253580 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Ultra-deep bulk RNA-seq from hESC-derived thyroid follicular cells

Project description:This study provides ultra-deep bulk RNA-seq data from human embryonic stem cell (hESC)-derived thyroid follicular cells. The NKX2-1GFP+ population was isolated using fluorescence-activated cell sorting (FACS) and subjected to RNA extraction. Library preparation was performed using the Ovation Solo RNA-seq System, followed by high-output sequencing on the Illumina HiSeq 1500 platform. Each sample generated approximately 400 million reads. These data enable transcriptomic analysis of differentiated thyroid lineage cells exposed to IFN-α and IFN-γ.

2025-08-13 | GSE295120 | GEO

Comprehensive proteomics analysis to distinguish follicular adenoma and follicular thyroid carcinoma from indeterminate thyroid nodules.Comprehensive proteomics analysis to distinguish follicular adenoma and follicular thyroid carcinoma from indeterminate thyroid nodules

Project description:In this study, a total of 202 formalin fixed paraffin embedded (FFPE) thyroid tissues including 62 of benign nodules, 72 of follicular adenoma, and 68 of follicular thyroid carcinoma were used for the analyses. Here, through global FFPE proteomic analysis, proteins differentially expressed by group were identified and quantitative analysis was performed by LC-MS/MS.

2025-10-13 | PXD043154 | Pride

Comparison of gene expression between follicular thyroid adenoma and follicular thyroid carcinoma

Project description:Follicular thyroid tumours were investigated using global gene expression analysis. Aim of this study was the identification of new markers for follicular thyroid carcinoma. Keywords: cell type comparison Gene expression analysis of 4 follicular thyroid adenomas, 4 follicular thyroid carcinomas, and 4 microinvasive follicular thyroid carcinomas.

2009-02-27 | E-GEOD-15045 | biostudies-arrayexpress

Transcription profiling of radiation-induced follicular thyroid adenomas with sporadic follicular thyroid adenomas

Project description:transcriptome comparison of radiation-induced follicular thyroid adenomas with sporadic follicular thyroid adenomas

2005-11-04 | E-MEXP-484 | biostudies-arrayexpress

Comparison of gene expression between follicular thyroid adenoma and follicular thyroid carcinoma

2009-02-28 | GSE15045 | GEO

Proteotypic differences of follicular-patterned thyroid neoplasms

Project description:The diagnosis of follicular-patterned thyroid tumors such as follicular adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors by proteomic analysis.

2022-09-07 | PXD036553 |

Transcription profiling of human benign and malignant thyroid follicular neoplasi

Project description:Differentiation between Follicular Thyroid Carcinoma and Follicular Thyroid Adenoma based on their genetic signature

2005-01-01 | E-MEXP-97 | biostudies-arrayexpress

A two miRNA classifier differentiates follicular thyroid carcinomas from follicular thyroid adenomas

Project description:The inherent diagnostic limitations of thyroid fine needle aspiration (FNA), especially in the “indeterminate” category, can be partially overcome by molecular analyses. We aimed at the identification of miRNAs that could be used to improve the discrimination of indeterminate FNAs. miRNA expression profiling was performed for 17 follicular carcinomas (FTCs) and 8 follicular adenomas (FAs). The microarray results underwent cross-comparison using three additional microarray data sets. Candidate miRNAs were validated by qPCR in an independent set of 32 FTCs and 46 FAs. Sixty-eight differentially expressed miRNAs were identified. Thirteen miRNAs could be confirmed by cross comparison. A two-miRNA-classifier was established improving the diagnostic applicability and resulted in a sensitivity of 82% and a specificity of 49%. We present a classifier that has the potential to be successfully evaluated in cytology material for its capability to discriminate (mutation negative) indeterminate cytologies and thereby improving the pre-surgical diagnostics of thyroid nodules. miRNA expression profiling was performed for 17 follicular carcinomas (FTCs) and 8 follicular adenomas (FAs). All samples are independent, coming from different patients.

2014-10-07 | E-GEOD-62054 | biostudies-arrayexpress

Transcriptional profiles of induced thyroid follicular like cells.

Project description:Defined transcription factors can direct changes in programmed cell fate to give rise to various cell lineages, in contrast to the one-way street during development. Direct lineage conversions from fibroblasts occur in all the three germ layer derivatives- neurons, cardiomyocytes, and hepatocytes. However, direct reprogramming of thyroid lineages has not been accomplished yet. Previous studies demonstrated that exogenous Pax8 and Nkx2.1, which are indispensable for proper thyroid development, enable the pluripotent stem cell-derived definitive endoderm to differentiate into functional thyroid follicular cells. This raised the question whether a cocktail of thyroid development-related transcription factors can directly convert fibroblasts into thyroid follicular cells in vitro. In the current study, we found that induction of defined transcription factors is able to generate thyroid follicular cells from fibroblasts. Further, Pax8, Nkx2.1, Foxe1, Oct3/4, Sox2, Klf4, and c-Myc were sufficient to generate thyroglobulin (Tg)+/EpCAM+ colonies from mouse embryonic fibroblasts, and these colonies built three-dimensional (3D) follicular-like structures in matrigel-based 3D culture. These induced thyroid follicular-like cells, named iTF cells, were morphologically and transcriptionally similar to those of the adult thyroid. Finally, iTF cells were transplantable into the kidney capsule, and formed huge follicular structures in vivo as well. Our study therefore reveals that thyroid lineage can be directly derived from fibroblasts in a single step, and contributes to rapid translational research on human thyroid diseases such as hyperthyroidism.

2016-09-30 | GSE76266 | GEO

miRNA high-throughput sequencing of follicular thyroid tumors

Project description:We report data obtaibed from high-throughput sequencing of small RNAs in 20 samples of follicular thyroid tumors. We analyzed a total of 4.7±1.5million reads per sample with 3 different pipelines. The main goal was to evaluate the usefulness of next generation sequencing in small RNA profiling and the concordance of its results with microarrays and qPCR. Additionally we verified published follicular thyroid tumor biomarkers in the set of our samples. Small RNA expression profiling with High Throughput Sequencing of 20 thyroid tumor samples, performed on an Illumina HiScan-SQ.

2014-03-07 | E-GEOD-39112 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data