Project description:To study the effects of bacterial infection upon gene expression changes in flounder liver fish were artificially "infected" by injection with a commercial water-based vaccine containing killed Aeromonas salmonicida, the bacterium responsible for furunculosis, which, as its name describes, presents as external lesions (furuncles, "lumps"). This disease occurs in flounder as well as in salmonids. Flounders were treated by intraperitoneal injection with 1ml/kg Furunculosis vaccine (Schering-Plough, Aquavac Furovac 5, batch number FNM/C/007). 30 control fish were injected with 1% saline. Animals were then maintained unfed in static aerated sea water tanks in a constant temperature containment aquarium. Water was renewed every 2 days. After 1, 2, 4, 8, and 16 days, 6 vaccine treated and 6 saline control fish were removed, killed by a blow to the head and samples of liver tissue were immediately homogenized in TriReagent prior to gene expression profiling. Microarray experiments consisted an individual array for each of 4 or 5 fish from each timepoint and each treatment compared with an artificial reference sample.
Project description:To investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae by co-culturing P. multocida in the conditioned media prepared from A. caviae
Project description:Vibrio cholerae is a Gram-negative bacterium that can cause pandemic cholera in humans. Meanwhile, the aquaculture environment heavy metal pollution situation is serious. In this study, the growths of the V. cholerae isolates recovered from commonly consumed fish were measured for the first time in sublethal concentrations of heavy metals.
Project description:We employed a transgenic strain of a small aquarium fish medaka (Oryzias latipes) that overexpresses a malignant melanoma driver gene. In this model, melanoma develops with 100 % penetrance. Using the medaka malignant melanoma model, we tested whether cisplatin and trametinib are able to suppress the transcriptomic changes induced by the transgene.
Project description:The discus fish (Symphysodon aequifasciatus) is an ornamental fish, which occupies important position on the freshwater aquarium trade. We built two cDNA libraries from an adult male brain and an adult female brain, and performed RNA-sequencing for identifying sex-biased candidate genes , a total number of 40209 non-redundant genes (unigenes) were obtained, of which 250 unigenes were significant overexpressed in the male brain, and 436 unigenes were significant overexpressed in the female. A total of 439 miRNAs were identified, of which 60 miRNAs were differentially expressed between male brain and female brain. These results can provide important evidence for better understanding the molecular mechanisms of the brain's amphoteric dimorphism in discus fish
Project description:Hepatic gene expression profiling in male Zebrafish in response to estradiol exposure and aquatic xenobiotics 17alpha-ethynylestradiol, Bis(2-ethylhexyl) phthalate and nonylphenol. Male zebrafish (Danio rerio) were purchased from commercial fish farm (Euroaquarium Spa Bologna, Italy). Approximately one hundred and twenty fish were received and divided evenly between three 80 l glass aquaria. Each aquarium was individually heated
Project description:High-throughput proteomics was used to determine the role of the fish liver in defense responses to bacterial infection, done using a rainbow trout (Oncorhynchus mykiss) model following infection with Aeromonas salmonicida, the causative agent of furunculosis. The vertebrate liver has multifaceted roles in innate immunity, metabolism, and growth; we hypothesize this tissue serves a dual function in supporting host defense in parallel to metabolic adjustments that promote effective immune function. While past studies have reported mRNA responses to A. salmonicida in salmonids, the impact of bacterial infectionon the liver proteome remains uncharacterized in fish.
Project description:This study was performed to validate the newly developed CGP Atlantic cod 20K oligonucleotide microarray. Atlantic cod (Gadus morhua) received an intraperitoneal injection of either formalin-killed, atypical Aeromonas salmonicida (Asal) or PBS and transcriptional profiles of spleen tissues from Asal-injected fish were compared to those from pre-injection control fish and PBS-injected control fish. Gene expression profiles resulting from this study were compared to those from suppression subtractive hybridization library studies, that were previously performed on the same samples, and to literature. Gene expression patterns of single genes were confirmed by QPCR analysis. This study has shown that the newly developed CGP Atlantic cod 20K oligo microarray platform is a valuable tool for cod genomic research.
Project description:Helicobacter pylori has a very plastic genome, reflecting its high rate of recombination and point mutation. This plasticity promotes divergence of the population by the development of subclones and presumably enhances adaptation to host niches. We have investigated the genotypic and phenotypic characteristics of two such subclones isolated from one patient as well as the genetic evolution of these isolates during experimental infection. Whole-genome genotyping of the isolates using DNA microarrays revealed that they were more similar to each other than to a panel of other genotyped strains recovered from different hosts. Nonetheless, they still showed significant differences. The genomic evolution of both isolates during the infection of conventionally raised and germ-free mice was monitored over the course of 3 months. The Cag PAI-positive isolate was also surveyed after a 10 month colonization of conventionally raised transgenic animals (n = 9 mice). Microarray analysis of the Cag PAI and sequence analysis of the cagA, recA, and 16S rRNA genes disclosed no changes in recovered isolates. Together, these results reveal that the H. pylori population infecting one individual can undergo significant divergence, creating stable subclones with substantial genotypic and phenotypic differences. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set
2005-10-20 | GSE3478 | GEO
Project description:Aeromonas salmonicida recovered during medical leech therapy
