Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA141717 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [ChIP-seq]
| PRJNA141713 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE27967: ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [ChIP-seq] GSE27969: ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [mRNA profiling] Refer to individual Series
2011-03-21 | E-GEOD-27970 | biostudies-arrayexpress
Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
2004-01-02 | GSE887 | GEO
Project description: Not available
| S-EPMC8784167 | biostudies-literature
Project description: Not available
| S-EPMC11335981 | biostudies-literature
Project description:H3K27me3 is a histone modification associated with transcriptional repression that plays a pivotal role in differentiation and development in plants and animals. H2A.Z, a histone variant of H2A, is often colocalized with H3K27me3 in Arabidopsis. Previous genome-wide studies have raised the possibility that H2A.Z contributes to H3K27me3 homeostasis in plants. To examine the contribution of H2A.Z to H3K27me3, we utilized hta9 hta11, a plant line which is defective in two of three genes that code for H2A.Z and has been previously characterized as an H2A.Z-depletion mutant. Surprisingly, ChIP-seq analysis of H2A.Z enrichment revealed that hta9 hta11 plants exhibit a nearly indistinguishable genic H2A.Z enrichment pattern relative to wild type with few genes that are enriched or depleted for H2A.Z. Mass spectrometry measurement of histone protein abundance in hta9 hta11 plants in conjunction with our ChIP-seq analysis indicates that the chromatin of hta9 hta11 plants exhibits altered relative abundance of H2A.Z variants rather than a global depletion of H2A.Z. In contrast, ChIP-seq analysis of H3K27me3 reveals that hta9 hta11 plants exhibit a robust global reduction in H3K27me3 enrichment, and numerous genes are identified that exhibit altered levels for H3K27me3. In addition, hta9 hta11 plants exhibit differential expression of thermoresponsive genes that have previously been shown to be dependent on H2A.Z. Our combined analyses indicate that specific H2A.Z isoforms play distinct roles in chromatin composition and gene expression in Arabidopsis, particularly with regards to H3K27me3 homeostasis.
2026-08-03 | PXD058289 | Pride
Project description:Sex differences in liver gene expression are dictated by sex-differences in circulating growth hormone (GH) profiles. Presently, the pituitary hormone dependence of mouse liver gene expression was investigated on a global scale to discover sex-specific early GH response genes that might contribute to sex-specific regulation of downstream GH targets and to ascertain whether intrinsic sex-differences characterize hepatic responses to plasma GH stimulation. RNA expression analysis using 41,000-feature microarrays revealed two distinct classes of sex-specific mouse liver genes: genes subject to positive regulation (class-I) and genes subject to negative regulation by pituitary hormones (class-II). Genes activated or repressed in hypophysectomized (Hypox) mouse liver within 30-90min of GH pulse treatment at a physiological dose were identified as direct targets of GH action (early response genes). Intrinsic sex-differences in the GH responsiveness of a subset of these early response genes were observed. Notably, 45 male-specific genes, including five encoding transcriptional regulators that may mediate downstream sex-specific transcriptional responses, were rapidly induced by GH (within 30min) in Hypox male but not Hypox female mouse liver. The early GH response genes were enriched in 29 male-specific targets of the transcription factor Mef2, whose activation in hepatic stellate cells is associated with liver fibrosis leading to hepatocellular carcinoma, a male-predominant disease. Thus, the rapid activation by GH pulses of certain sex-specific genes is modulated by intrinsic sex-specific factors, which may be associated with prior hormone exposure (epigenetic mechanisms) or genetic factors that are pituitary-independent, and could contribute to sex-differences in predisposition to liver cancer or other hepatic pathophysiologies.
2010-01-27 | GSE17644 | GEO
Project description:To understand the mechanisms through which JunB regulates Tregs-mediated immune regulation, we examined the global gene expression profiles in the JunB WT and KO Tregs by performing RNA sequencing (RNA-seq) analysis.
2019-05-07 | GSE129060 | GEO
Project description: Not available
| S-EPMC5321798 | biostudies-literature