Project description:This SuperSeries is composed of the following subset Series: GSE25438: Gene expression data in murine nasal mucosa following nanoemulsion adjuvant exposure GSE25485: Gene expression data in Bone Marrow Derived Dendritic Cells (BMDC) following nanoemulsion adjuvant exposure Refer to individual Series
Project description:Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant.
Project description:Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant. 8 week old female CD-1 mice were nasally treated with 5 microliters/nare of either 20% (v/v) naomeulsion (W805EC) or PBS. Nasal epithelium was harvested immediately post-mortem at either 6 (6 hr) or 24 hours (24 hr) following treatment. The tissue was placed in OTC and frozen by immersion in liquid nitrogen. Total RNA was extracted per sample using RNA easy (Qiagen).
Project description:Nanoemulsion adjuvant affects immune gene expression in dendritic cells. Microarray was used to asses global changes in gene expression in JAWS II dendritic cells upon treatment with nanoemulsion (NE) adjuvant W805EC
Project description:Nanoemulsion adjuvant affects immune gene expression in dendritic cells. Microarray was used to asses global changes in gene expression in JAWS II dendritic cells upon treatment with nanoemulsion (NE) adjuvant W805EC JawsII cell were incubated with NE formulation for 6 and 24 hours, and RNA was extracted for Affymetrix hybridization
