Project description:Endothelial cells of blood vessel playing a key role in the metastasis of tumor cells particularly the brain microvascular endothelial cells in the brain-tropic metastasis of lung tumor cells. Currently, studies towards the influence contributed by tumor cells to the alteration of endothelial cells have been extensively conducted by contrast studies for the alteration of gene-expression signature of tumor cells under the influence of endothelial cells remains poorly characterized, thus in this study the transcriptome of the human small-cell lung cancer cell NCI-H209 was sequenced via RNA-Seq after conditioned by human brain microvascular endothelial cell (HBMEC) for characterizing the alteration of gene-expression signature.
Project description:To evaluate the impact of mTOR activation by Tsc2KD in MVPC on the microvascular endothelial cell population we utilized single cell transcriptomic analysis of fractionated lung tissue. Mouse lung samples were pooled and cell sorting was 2 days and 10 weeks post tamoxifen induction. Following annotation of the original 28 lung subclusters, capillary MVEC were localized to 7 subclusters. Temporal comparison of the MVEC populations demonstrated that in WT the population was transcriptionally stable from 2 days to 10 weeks postinduction, as would be expected during angiostasis. In contrast lung MVEC from the mice with mTOR+ Tsc2KD MVPC demonstrated significant increases in gene expression at 10 weeks relative to day 2 post-induction. Increased gene expression was related to mesenchymal stem cell differentiation, microvascular endothelial differentiation, mTOR activation, autophagy and apoptosis. These detailed complementary analyses identify mechanistic consequences of Tsc2KD and mTOR dysregulation in MVPC.
Project description:The purpose is to obtain samples for mRNA analysis in primary human lung microvascular endothelial cells treated with universal interferon alpha beta or interferon gamma.
Project description:Using RNA-seq we report changes in gene expression, over a 24 hour time course, in human lung microvascular endothelial cells subjected to 10 Gy X-irradiation.
Project description:Gene expression study of DSG2 silenced human microvascular endothelial cells Affymetrix GeneChip Human Genome U133 Plus 2.0 Array were used to examine differentially expressed genes between normal microvascular endothelial cells and DSG2 silenced microvascular endothelial cells
