Project description:Comparison of gene expression level of 3T3-L1, PMEF and ES cell derived adipocytes to eWAT samples. We used microarray to investigate the gene expressional differences between different cellular models of adipocyte differentiation vs. in vivo eWAT tissues
Project description:Inhibition of pre‑rRNA transcription by BMH21 (12 h) in ex vivo mouse testicular single‑cell suspensions, followed by FACS isolation of pachytene spermatocytes (Hoechst 33342) and RNA‑seq. This dataset is designed to assess whether perturbing pre‑rRNA accumulation within the XY body affects the transcriptional landscape of pachytene spermatocytes, particularly regarding meiotic sex chromosome inactivation (MSCI). Comparison of BMH21‑treated vs control cells identifies differentially expressed autosomal and sex‑linked genes, providing insights into the role of nucleolar activity in sex chromosome regulation during male meiosis.
