Project description:We collected whole genome testis expression data from hybrid zone mice. We integrated GWAS mapping of testis expression traits and low testis weight to gain insight into the genetic basis of hybrid male sterility.
Project description:We conducted global proteome and phosphoproteome profiling of vehicle- and everolimus-treated tumors from 17 mice that were allografted with spontaneously developed mammary tumors from Brca1co/coMMTV-Cre mice using LC-MS/MS. The Proteome Discoverer analysis identified 9,625 proteins having two unique sibling peptides and more and 35,142 phosphopeptides with a peptide- and protein-level FDR of 1% using SEQUEST-HT algorithm. We defined everolimus-sensitive and resistant tumors based on proteome and phosphoproteome data, and identified protein and phosphopeptide signatures defining each cluster. Pathway enrichment analysis of these signatures further revealed that leukotriene-neutrophil activation pathways were upregulated in everolimus-resistant tumors.
