Project description:Polysome-fraction RNA sequencing of HEK293 cells treated with vehicle control or minocycline. Cytoplasmic lysates were separated into monosome, light-polysome, and heavy-polysome fractions, ERCC spike-ins were added after fractionation and before RNA extraction, and fraction RNA was sequenced to quantify transcript ribosome-load state. The processed matrix reports control fraction distributions, fraction-enrichment classifications, polyEN, and mean ribosome load (MRL).
Project description:Transcriptional profiling of Cytoplasmic mRNA and Heavy polysome-associated mRNAs extracted from U251 Cells treated with control siRNA or siRNA targeting eIF3e . Goal was to determine the effect of eIF3e knockdown on translation of specific mRNAs.
Project description:Subcutanesouly tumors from both Bmal1+/+ and Bmal1-/- mice were used to isolated stromal vascular fractions (SVF). Tumor cells with GFP+ signals were exclusive. Remain GFP- cells were collected to do RNAseq.
Project description:SILAC based protein correlation profiling using size exclusion of protein complexes derived from Mus musculus tissues (Heart, Liver, Lung, Kidney, Skeletal Muscle, Thymus)
