Project description:Differentiation of hemopoietic stem cells into granulocytes is characterized by distinct changes in the transcriptome. We analyzed mRNA expression in primary murine myeloid cells at four successive stages of hemopoietic differentiation; Lin- Sca1+ cKit+ stem/progenitor cells (LSKs), promyelocytes, myelocytes and granulocytes. Using fluorescence‚Äìactivated cell sorting, we isolated primary murine myeloid cells at four successive stages of hemopoietic differentiation; Lin- Sca1+ cKit+ stem/progenitor cells (LSK), promyelocytes, myelocytes and granulocytes.

2014-05-14 | E-GEOD-57622 | biostudies-arrayexpress

Mus musculus

Project description:Global microRNA profiling of mouse hemopoietic stem cells (LSKs), promyelocytes, myelocytes and granulocytes.

| PRJNA247241 | ENA

Global microRNA profiling of mouse hemopoietic stem cells (LSKs), promyelocytes, myelocytes and granulocytes.

Project description:This study sought to determine the dynamic changes of miRNA expression during mouse granulopoiesis. We not only performed analyses of miRNA expression levels in whole cells but also analyzed purified nuclear and cytoplasmic cell fractions to profile miRNA subcellular localization. qRT-PCR analysis of miRNAs was performed on whole cell, nuclear and cytoplasmic RNAs extracted from mouse hemopoietic stem cells (LSKs), promyelocytes, myelocytes and granulocytes. 100 ng of RNA was reversed transcribed using the Taqman miRNA Reverse Transcription Kit and Megaplex RT Primers rodent pool A and B (Life Technologies). Complementary DNA (cDNA) was amplified using a TaqMan rodent microRNA A and B Array v2.0 (Life Technologies) with TaqMan Universal PCR Master Mix on an ABI 7900HT Sequence Detection System.

2014-05-14 | E-GEOD-57623 | biostudies-arrayexpress

E-GEOD-47422

Project description:Expression data from TFIIA-non-cleavable mouse fetal liver hematopoietic stem cells

2015-07-14 | E-GEOD-47422 | ExpressionAtlas

E-GEOD-45826

Project description:Expression data from sorted control and Pygo2-null mouse mammary stem/basal cells

2015-07-13 | E-GEOD-45826 | ExpressionAtlas

E-GEOD-58960

Project description:Expression data from embryonic stem cells differentiation

2015-08-04 | E-GEOD-58960 | ExpressionAtlas

Mus musculus hematopoietic stem cell

Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.

2009-05-20 | GSE16139 | GEO

E-GEOD-37859

Project description:Gene expression profile of mouse induced neural stem cells

2015-09-29 | E-GEOD-37859 | ExpressionAtlas