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Escherichia coli BW25113

ABSTRACT: Expression Profiling of E. coli K-12 BW25113 in Minimal Media with Different Carbon Sources

PROVIDER: PRJNA256161 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1525100_1.fastq.gz	Fastqsanger.gz
	SRR1525100_2.fastq.gz	Fastqsanger.gz
	SRR1525101_1.fastq.gz	Fastqsanger.gz
	SRR1525101_2.fastq.gz	Fastqsanger.gz
	SRR1525102_1.fastq.gz	Fastqsanger.gz

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Gene expression for Escherichia coli BW25113 exponential growth on minimal medium with eight different carbon sources: Glucose, Galactose, Glycerol, Gluconate, Fructose, Pyruvate, Succinate, Acetate.

Project description:This dataset contains the gene expression signature in triplicates of Escherichia coli BW25113 growing exponentially on eight different environments: minimal medium supplemented with 5 g/L of Glucose, Galactose, Glycerol, Gluconate, Fructose, Pyruvate, Succinate or Acetate as the sole carbon source.

2015-10-07 | E-MTAB-3392 | biostudies-arrayexpress

Escherichia coli BW25113

Project description:Expression Profiling of E. coli K-12 BW25113 in Minimal Media with Glucose and Amino Acid Supplementations

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E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells

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BW25113 Rif pre-treated persister cells vs. stationary phase BW25113 wild-type

Project description:E. coli K-12 BW25113 persister cells generated via rifampicin pre-treatment relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL.

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Escherichia coli transcriptome reorganization caused by nutritional changes

Project description:Escherichia coli K-12 BW25113 were cultured in the LB and M63 media. Exponetially growing populations were collected for RNAseq. The culture medium-induced transcriptional changes were analyzed.

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BW25113 H202 and delta rpoS persister cells vs. stationary phase BW25113 wild-type

Project description:E. coli K-12 BW25113 persister cells generated via H202 pre-treatment and deletion of rpoS, relative to BW25113 wild-type stationary phase gene expression. Persister cells were generated following exposure to ampicillin 20 ug/mL.

2012-04-03 | GSE34028 | GEO

Transcriptional responses of Escherichia coli rpoS- BW25113 vs. wild-type BW25113 under 15% ethanol shock in log phase

Project description:RpoS, an alternative sigma factor, is critical for stress response in Escherichia coli.RpoS also acts as a global regulator for stress control of gene expression, and actually dose so in log stage and stationary stage. To further understand the effect of environmental stresses on in ethanologenic strains, DNA microarrys was used to analyze the expression profiles of E. coli and its rpoS mutant strain. BW25113（rpoS-）and BW25113 were selected at log stages and stationary stage of early development for RNA extraction and hybridization on Affymetrix microarrays. To that end, we hand-selected BW25113 and BW25113（rpoS-） according at different treatments: BW25113（rpoS-）at log stage (BW25113(rpoS-) log), BW25113 at log stage (BW25113 log),BW25113（rpoS-）atstationary stage ( BW25113(rpoS-) stationary), BW25113 at stationary stage ( BW25113 stationary)

2010-07-20 | E-GEOD-17526 | biostudies-arrayexpress

Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis

Project description:E. coli isolates from different CF patients demonstrate increased growth rate when grown with glycerol, a major component of fecal fat, as the sole carbon source compared to E. coli from healthy controls. CF and control E. coli isolates have differential gene expression when grown in minimal media with glycerol as the sole carbon source. While CF isolates display a growth promoting transcriptional profile, control isolates engage stress and stationary phase programs, which likely results in slower growth rates.

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Expression Profiling of E. coli K-12 BW25113 in Minimal Media with Different Carbon Sources

Project description:mRNA sequencing was performed on E. coli K-12 BW25113 grown on Glucose, Pyruvate, Fumarate, and Acetate.

2017-02-14 | GSE59759 | GEO

E. coli BW25113 ompA mutant vs wild type biofilm and E. coli BW25113 ompA/pCA24N_ompA vs ompA/pCA24N suspension cells

Project description:This Series involves two studies: 1) The gene expression of E. coli K-12 BW25113 ompA mutant strain vs. wild type strain glasswool biofilm cells and E. coli K-12 BW25113 ompA mutant vs. wild type polystyrene biofilm cells. 2) The gene expression of E. coli BW25113 ompA/pCA24N_ompA vs. ompA/pCA24N suspension cells. Strains: E. coli K-12 BW25113 wild type, ompA mutant Medium: LB Cell type: Biofilm cells grown on glasswool and polystyrene surfaces Time: 15 h Temperature: 37C Strains: BW25113 ompA/pCA24N_ompA and ompA/pCA24N Medium: LB Time: 7 h Temperature: 37C Cell type: suspension cells, induced by 0.1 mM IPTG

2010-11-03 | E-GEOD-14064 | biostudies-arrayexpress

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