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Bacillus subtilis subsp. subtilis str. 168

ABSTRACT: Altered germination properties of Bacillus subtilis 168 strains

PROVIDER: PRJNA268543 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR1663457_1.fastq.gz	Fastqsanger.gz
	SRR1663457_2.fastq.gz	Fastqsanger.gz
	SRR1663467_1.fastq.gz	Fastqsanger.gz
	SRR1663467_2.fastq.gz	Fastqsanger.gz

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RNA-seq established gene expression data to determine the effects of various stresses on Bacillus subtilis str.168

Project description:To explore the effects of different stress conditions on Bacillus subtilis str.168, a selection of conditions were applied to the organism and RNA-seq data gathered. A matrix of gene counts was produced as a basis for further analysis into the transcription profiles of Bacillus subtilis str.168.

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Expression analysis of sporulating Bacillus subtilis strain 168 deleted for prpE gene

Project description:Investigation of whole genome gene expression level changes in sporulating Bacillus subtilis 168 delta-prpE mutant, compared to the wild-type strain. The mutation engineered into this strain results in impaired germination of spores. A six chip study using total RNA extracted from three separate wild-type cultures of sporulating Bacillus subtilis 168 and three separate cultures of sporulating mutant strain, Bacillus subtilis 168 delta-prpE, in which prpE (yjbP BSU11630) gene coding for a protein phosphatase is deleted entirely. Each chip consists of four fields able to measure the expression level of 4,104 genes from Bacillus subtilis subsp. subtilis strain 168 NC_000964 with eight 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.

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Project description:In this work we present proteomic analysis of Bacillus subtilis 168 trp+ (BSB1) during germination and spore outgrowth. Samples were collected from 14 different time points (from 0 to130 minutes) after inoculation of spores into germination medium. In summary, 2,195 proteins were identified and clustered according to their expression kinetics. Results revealed five specific clusters that were functionally analyzed.

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Bacillus subtilis 168 moenomycin stimulon

Project description:Transcriptional response of Bacillus subtilis to moenomycin in wild-type 168. Bacillus subtilis 168, WT (-MOE) vs. WT (+MOE). The experiment was conducted in triplicate using three independent total RNA preparations. Untreated samples were labeled with Alexa Fluor 555 and moenomycin treated samples were labeled with Alexa Fluor 647.

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Project description:Identification of the specific WalR (YycF) binding regions on the B. subtilis chromosome during exponential and phosphate starvation growth phases. The data serves to extend the WalRK regulon in Bacillus subtilis and its role in cell wall metabolism, as well as implying a role in several other cellular processes. For each sample analyzed in this study three biological replicates were performed. Three different samples were taken from a strain expressing the WalR-SPA protein as well as from wild-type (168) without a tagged WalR. Samples were taken from exponentially growing cells in low phosphate medium (LPDM) as well as from phosphate-limited cells (T2). Each sample compares ChIP DNA vs. Total DNA from the same cells.

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