Project description:We constructed three small RNA libraries from embryos at 5, 6 and 7 post-oviposition (hpo) of Bactrocera dorsalis for deep sequencing. The data analysis revealed 147 known and 103 novel miRNAs from these libraries.
Project description:Four developmental stage small RNA libraries including Eggs, Larvae, Pupae and Adults were constructed, where a lot of known miRNAs were identified and many novel miRNAs were predicted. Comparison of their expression profiles in the four libraries suggested that Bactrocera dorsalis miRNAs are dynamically regulated throughout the life cycle.Further analysis of the expression and function of these miRNAs could increase our understanding of regulatory networks in the insect and lead to novel approaches to its control.
Project description:Comprehensive RNA sequencing was performed on a laboratory colony of B. dorsalis with a focus on attempting to capture as many genes in the sequencing from throughout the entire developmental life history. De novo assembly and analysis of the resulting sequence One sample each for the egg, larvae, pupae, adult male, adult female and mated female life stages was sequenced.
Project description:<p>Depletion of gut commensal bacteria significantly reduced nicotinic acid (NA) levels in the hemolymph and ovaries of Bactrocera dorsalis, leading to impaired ovarian development. This inhibition was reversed by exogenous NA supplementation. We identified Enterobacter hormaechei as a key NA-producing gut symbiont. Recolonization with E. hormaechei restored NA levels in both the hemolymph and ovaries, thereby rescuing ovarian development in B. dorsalis.</p>
