Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA286736 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:To characterize the progenitor-enriched CD44+NGFR+ cells present in the crypt and surface regions of the tonsil, we used an Illumina array system to analyze the transcriptome of these cells isolated from the tonsillar crypt and surface regions of three different individuals as well as the total epithelial (CD45-CD31-) populations obtained from two of these. To enrich for epithelial progeniors in human palatine tonsil tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from palatine tonsils using fluorescence-activated cell sorting (FACS). The resultant CD45-CD31- population was further fractionated into four subpopulations using antibodies against CD44 and NGFR. Based on the immunohistochemical phenotype and in vitro functional assays, CD44+NGFR+ subpopulations were identified as epithelial progenitor-enriched subsets. Microarray profiling was used to derive gene expression signatures of CD45-CD31- subsets (total epithelial-enriched) and CD45-CD31-CD44+NGFR+ subpopulations (progenitor-enriched).
2015-10-29 | E-GEOD-69767 | biostudies-arrayexpress
Project description:Gene expression profiles of normal mouse mammary cell subpopulations FACS sorted based on expression of biomarkers
| PRJNA121805 | ENA
Project description:Gene expression profiles of normal human mammary cell subpopulations FACS sorted based on expression of CD49f and EpCAM
| PRJNA117321 | ENA
Project description:As a starting point for dissecting the cellular heterogeneity of astrocytes in the adult brain, we focused our initial studies on the olfactory bulb (OB) and combined an Aldh1l1-GFP reporter mouse with candidate cell surface markers to isolate astrocyte sub-populations using FACS analysis. To this end, we screened a panel of cell surface antigens for their ability to divide the Aldh1l1-GFP astrocyte population within the OB into distinct sub-populations. Candidate cell surface markers were selected based on previously published gene expression profiling data from our lab and others and antibody availability. Different subpopulations from different brain regions were profiled for gene expression.
2017-01-16 | GSE72826 | GEO
Project description:The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyse the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signalling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. </br></br> The Lipidomic LC-MS assay is reported in the current study MTBLS631. </br> The General LC-MS assay is reported in MTBLS629. </br> The CE-MS assay is reported in MTBLS633. </br> The GC-MS assay is reported in MTBLS634. </br></br> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS629' target='_blank'><span class='label label-success'>MTBLS629</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS633' target='_blank'><span class='label label-success'>MTBLS633</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS634' target='_blank'><span class='label label-success'>MTBLS634</span></a>
2018-11-06 | MTBLS631 | MetaboLights
Project description:The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyse the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signalling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. </br></br> The CE-MS assay is reported in the current study MTBLS633. </br> The General LC-MS assay is reported in MTBLS629. </br> The Lipidomic LC-MS assay is reported in MTBLS631. </br> The GC-MS assay is reported in MTBLS634. </br></br> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS629' target='_blank'><span class='label label-success'>MTBLS629</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS631' target='_blank'><span class='label label-success'>MTBLS631</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS634' target='_blank'><span class='label label-success'>MTBLS634</span></a>
2018-11-06 | MTBLS633 | MetaboLights
Project description:The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyse the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signalling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. </br></br> The General LC-MS assay is reported in the current study MTBLS629. </br> The Lipidomic LC-MS assay is reported in MTBLS631. </br> The CE-MS assay is reported in MTBLS633. </br> The GC-MS assay is reported in MTBLS634. </br></br> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS631' target='_blank'><span class='label label-success'>MTBLS631</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS633' target='_blank'><span class='label label-success'>MTBLS633</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS634' target='_blank'><span class='label label-success'>MTBLS634</span></a>
2018-11-06 | MTBLS629 | MetaboLights
Project description:The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyse the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signalling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. </br></br> The GC-MS assay is reported in the current study MTBLS634. </br> The General LC-MS assay is reported in MTBLS629. </br> The Lipidomic LC-MS assay is reported in MTBLS631. </br></br> The CE-MS assay is reported in MTBLS633. </br></br> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS629' target='_blank'><span class='label label-success'>MTBLS629</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS631' target='_blank'><span class='label label-success'>MTBLS631</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS633' target='_blank'><span class='label label-success'>MTBLS633</span></a>
2018-11-06 | MTBLS634 | MetaboLights
Project description:Here, we used a proteomics approach to identify novel chordoma-specific cell-surface protein markers. Four established chordoma cell lines (U-CH17P, U-CH17M, U-CH17S and U-CH11R) were analyzed by quantitative proteomics using a comprehensive organellar fractionation approach based on differential ultracentrifugation. A subtractive proteomics strategy was applied to identify proteins that are plasma membrane enriched. The expression profiles of these cell-surface proteins were validated across chordoma cell lines, patient surgical tissue samples, and normal tissue lysates. The essentiality of these candidates was evaluated using chordoma cell line growth in vitro.
2022-03-31 | MSV000089171 | MassIVE
Project description:The cellular heterogeneity of one patient derived orthotopic breast cancer xenograft model (PDBCX) was investigated using flow cytometry , combined with assessment of in vivo tumorigenicity and whole genome expression profiling. Epithelial cell adhesion molecule (EpCAM) was revealed as a highly specific cell surface marker of the human tumor cell population in both xenografts. Based on expression patterns observed in primary tumor tissue, SSEA-4 and CD24 were chosen as markers to further subdivide the luminal tumor cells into four subpopulations. FACS sorting was used to isolate four cell subpopulations. Results: In vivo tumorigenicity assay showed that SSEA-4+/CD24+ cells were non-tumorigenic, while the three other subpopulations were tumorigenic. Tumors resulting from the SSEA-4+/CD24- subpopulation of luminal cancer cells, did not express CD24, while tumors arising from the SSEA-4-/CD24-, and SSEA-4-/CD24+ populations both recapitulated the original tumor containing all four subpopulations. Whole genome expression analysis revealed distinct transcriptional profiles, and 44 genes were significantly differentially expressed when comparing the tumorigenic vs non-tumorigenic populations. Several interesting genes putatively suppressing the cancer cells ability to initiate tumors in vivo were upregulated in the non-tumorigenic population. We here show that tumor initiating cells within one primary tumor evidently included more than one phenotype. Furthermore, with respect to cell surface marker expression, one of the subpopulations produced tumors unlike both the originating cells, and the original tumor. Discussion: Our results imply that subpopulations from one primary tumor can give rise to dissimilar daughter tumors. These tumors may not necessarily respond to the same targeted treatment, and thereby represent a therapy escape mechanism. This study highlights that to remove the risk of breast cancer recurrence, inhibition of the molecules critical for driving the tumor progression in several tumor cell subpopulations might be essential Gene expression was measured in four cell subpopulations isolated from Patient derived human luminal-like breast cancer xenograft. Four replicates from three subpopulations and three replicates from one subpopulation.
2015-05-26 | E-GEOD-48384 | biostudies-arrayexpress