Project description:Gene-level analysis of gene expression in immortalized mouse keratinocyte lines that express or lack integrin alpha3beta1 The laminin-332-binding integrin alpha3beta1 is expressed highly in the epidermis, but its roles in regulating gene expression programs that promote normal or pathological skin remodeling have not been underexplored previously. In order to identify genes that are regulated by alpha3beta1 in keratinocytes, we used microarray approaches to identify alpha3beta1-dependent genes in mouse keratinocytes that were immortalized with SV40 large T antigen (i.e., MK cells).
Project description:Gene-level analysis of gene expression in immortalized mouse keratinocyte lines that express integrins alpha3beta1 and alpha9beta1 in different combinations. The integrins alpha3beta1 and alpha9beta1 are expressed highly in the epidermis, but their roles in regulating gene expression programs that promote normal or pathological wound healing have not been explored previously. In order to identify genes that are regulated by these integrins in keratinocytes, we used microarray approaches to identify integrin-dependent genes in mouse keratinocytes that were immortalized with SV40 large T antigen (i.e., MK cells).
Project description:Nucleolus-associated DNA was isolated from MEF cells before and after conditional knock-out of UBF and hybridized against genomic DNA in biological replicates. Two different types of immortalized MEF cells were used. MEFs were immortalized by genetic depletion of p53, iMEFs were immortalized by transfection of the SV40 Tt antigen.
