Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig
Project description:Large White and Meishan pigs were either non-treated or injected with mammalian 1-24 ACTH (Immediate Synachten, Novartis France) at the dose of 250 µg per animal. Pigs were sacrificed either immediately after capture from their home cage (non-treated animals) or 1 hour following ACTH injection. Adrenal glands were immediately collected from pigs and frozen on dry ice and then stored at -80°C until RNA isolation. Keywords: stress response, adrenal, gene expression, pig 47 samples
Project description:Miniature pig is a useful animal model to clarify the vital reaction and the molecular mechanisms. However, physiclogical response of pig model to sodium azide (AZIDE) stress is not reveal. To establishment of large animal model for evaluate toxic stress, whole blood of miniature pig were assessed with genomics. 7 month old clawn miniature pigs were fed AZIDE feeding. AZIDE dose of 300µg/kg, one hundredth of LD50 was given orally to the miniature pigs. Whole blood gene expression was measured at 0h, 6h and 24h after feeding.
Project description:Miniature pig is a useful animal model to clarify the vital reaction and the molecular mechanisms. However, physiclogical response of pig model to sodium azide (AZIDE) stress is not reveal. To establishment of large animal model for evaluate toxic stress, whole blood of miniature pig were assessed with genomics.
Project description:Minipigs are animal models widely used in biomedical studies due to their physiological and anatomical similarities to humans. However, a comprehensive resource for the Korean minipig (Sus scrofa) transcriptome remains unavailable. In this study, we constructed a de novo transcriptome of the Korean minipig using RNA-seq data obtained from ten tissues across ten samples. The final assembly comprised 57,085 coding transcripts with an average length of 3,075 nucleotides and an N50 of 4,258 nucleotides. In total, 65.4% of the transcripts were annotated, and biological functions were assigned. Transcript expression profiling and principal component analysis showed that samples clustered by tissue type, reflecting transcriptomic features shared across tissues. Comparative analysis demonstrated that the novel transcriptome assembly had contiguity and completeness comparable to those available for pig and minipig breeds. Overall, this study provides a comprehensive transcriptomic resource for the Korean minipig, facilitating further functional analyses.
Project description:Minipigs are animal models widely used in biomedical studies due to their physiological and anatomical similarities to humans. However, a comprehensive resource for the Korean minipig (Sus scrofa) transcriptome remains unavailable. In this study, we constructed a de novo transcriptome of the Korean minipig using RNA-seq data obtained from ten tissues across ten samples. The final assembly comprised 57,085 coding transcripts with an average length of 3,075 nucleotides and an N50 of 4,258 nucleotides. In total, 65.4% of the transcripts were annotated, and biological functions were assigned. Transcript expression profiling and principal component analysis showed that samples clustered by tissue type, reflecting transcriptomic features shared across tissues. Comparative analysis demonstrated that the novel transcriptome assembly had contiguity and completeness comparable to those available for pig and minipig breeds. Overall, this study provides a comprehensive transcriptomic resource for the Korean minipig, facilitating further functional analyses.